Abdominal Puncture
Abdominal Puncture - Related Videos
04:47
Cardiac Blood Collection via Subclavian Puncture in Mice
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2025
Subclavian cardiac puncture is a simple, reliable method for collecting terminal blood in mice. It is easy to operate and ensures high-purity samples.
11:53
Cecal Ligation Puncture Procedure
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The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.
13:32
Abdominal Exam IV: Acute Abdominal Pain Assessment
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2023
Source: Joseph Donroe, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT Abdominal pain is a frequent presenting concern in both the emergency department and the office setting. Acute abdominal pain is defined as pain lasting less than seven days, while an acute abdomen refers to the abrupt onset of severe abdominal pain with features suggesting a surgically intervenable process. The differential diagnosis of acute abdominal pain is broad; thus, clinicians must have a...
06:50
Abdominal Exam II: Percussion
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2023
Source: Alexander Goldfarb, MD, Assistant Professor of Medicine, Beth Israel Deaconess Medical Center, MA Medical percussion is based on the difference in pitch between the sounds elicited by tapping on the body wall. The auditory response to tapping depends on the ease with which the body wall vibrates, and is influenced by underlying organs, strength of the stroke, and the state of the body wall. There are three main medical percussion sounds: resonance (heard over lungs), tympany (heard over...
06:40
Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
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Cited by 56 •
2014
Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method. Current protocols to assess autophagy in vivo in the context of CLP-induced sepsis are presented here: A protocol for measuring autophagy using (GFP)-LC3 mice, and a protocol for measuring autophagosome formation by electron microscopy.
FAQs
The procedure can produce local pain-sensing and immune responses after protective tissues are breached. These responses may change locomotion, grooming, feeding, or other measured behaviors, so an observed behavioral shift may reflect the physical injury and its biological consequences rather than the subject’s baseline behavioral characteristics. This makes timing and interpretation of behavioral assessments especially important.
Puncture location and depth influence the extent and consistency of the controlled injury, which can affect the resulting pain-sensing and immune responses. If these features vary between subjects, behavioral differences may reflect procedural variation instead of treatment or genotype. Standardizing them therefore supports reproducible comparisons and helps researchers attribute outcomes to the intended experimental condition.
Breaching the abdominal wall activates local pain-sensing and immune responses. Those responses provide a biological pathway through which physical damage can influence actions such as movement, grooming, and feeding. In behavioral experiments, this connection allows the procedure to serve as a controlled challenge for examining how subjects respond to injury, stress, or infection-related conditions.
Researchers should keep the puncture location, penetration depth, and handling consistent across experimental groups. These features determine how comparable the induced injury is from one subject to another, while handling itself can contribute to behavioral effects. Consistent execution reduces unwanted variation and improves the ability to compare treatments, genetic conditions, or behavioral responses.
Post-procedure assessments can examine locomotion, grooming, feeding, and other behavioral measures selected for the study. Changes in these outcomes may indicate responses to the induced injury or related stress and infection challenges. Comparing the same measures across controlled experimental groups can reveal whether a treatment or genetic condition modifies the behavioral response.
This approach is useful when researchers need a standardized physical challenge for comparing responses to injury, stress, or infection-related conditions. It can support evaluations of treatments or genetic conditions by placing groups under a controlled injury-related context. Interpretation should distinguish procedure-related behavioral effects from broader phenotypes that exist independently of the puncture.