Oxygen Uptake

Oxygen uptake is the rate at which an organism absorbs and uses oxygen to support cellular energy production, making it a key indicator of metabolic demand. During respiration, oxygen moves into the body through respiratory surfaces, enters the circulation, and is delivered to tissues, where mitochondria use it to generate ATP. In behavioral research, measuring oxygen uptake helps relate activity, movement, thermoregulation, stress, and other behaviors to changes in energy expenditure. Comparing uptake across individuals or conditions can reveal how animals allocate energy and respond physiologically to their environment, supporting studies of performance, adaptation, and behavioral ecology.

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Research

JoVE EoE - Assay Techniques

Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids

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2025

This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.

Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria

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2025

This video describes a plate reader-based assay to measure mitochondrial calcium uptake in isolated mitochondria. The assay analyzes the fluorescence signal of a calcium-sensitive fluorescent dye to study the kinetics of mitochondrial calcium uptake and calcium overload.

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence

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Cited by 27 •

2011

We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.

Education

JoVE Science Education - Advanced Biology

Detecting Reactive Oxygen Species

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2023

Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...

Competence-Induced DNA Uptake in Staphylococcus aureus

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2025

Source: Cafini, F., et.al. Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus. J. Vis. Exp. (2017)This video demonstrates competence-induced DNA uptake in Staphylococcus aureus through natural transformation. A chloramphenicol-sensitive strain engineered to constitutively express a sigma factor is cultured under nutrient-limiting conditions to enhance competence, then incubated with donor DNA carrying a resistance gene. Successful gene transfer is confirmed by the...

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