Perfusion Imaging Analysis
Perfusion Imaging Analysis - Related Videos
12:29
Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling
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Cited by 18 •
2011
A MR imaging method to study the distribution of pulmonary blood flow under a variety of physiological conditions, in this case exposure to three different inspired oxygen concentrations: hypoxia, normoxia, and hyperoxia, is described. This technique utilizes human pulmonary physiology research techniques in an MR scanning environment.
10:16
Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging
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Cited by 25 •
2012
Visualization of the coronary vessels is critical to advancing our understanding of cardiovascular diseases. Here we describe a method for perfusing murine coronary vasculature with a radiopaque silicone rubber (Microfil), in preparation for micro-Computed Tomography (μCT) imaging.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
10:50
Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging
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Cited by 9 •
2013
We show here the in vivo insertion of an osmotic pump for constant local drug delivery and the creation of hindlimb ischemia in a mouse model. Moreover, the hindlimb vasculature is perfused with Microfil, a silicone radiopaque agent, to prepare for micro-computed tomography (micro-CT) imaging.
FAQs
Cerebral blood flow estimates how much blood reaches a tissue region, blood volume reflects the amount present, and transit time describes how long blood takes to pass through that region. Considering these measures together provides a more informative picture of circulation than relying on one value alone. In behavioral studies, regional patterns can be examined alongside differences in task performance.
Perfusion imaging analysis can use data acquired with a contrast agent, tracer, or blood-oxygenation signal. Because these approaches provide different signal bases, the resulting estimates depend on how the acquisition captures circulation-related information. The selected approach therefore shapes which perfusion measures can be derived and how confidently researchers can relate regional blood delivery to behavioral variation.
A relationship between a perfusion measure and behavioral performance shows that the variables vary together, but it does not by itself establish that altered blood delivery causes the behavior. Regional differences, individual differences, or other study factors may contribute to the pattern. Careful interpretation is especially important when examining complex outcomes such as attention, learning, emotion, or decision-making.
A typical workflow begins with acquiring brain images using a contrast agent, tracer, or blood-oxygenation signal. The data are then processed to generate quantitative perfusion maps, including measures such as cerebral blood flow, blood volume, or transit time. Researchers compare these regional values with behavioral performance to identify relationships between circulation and functions studied in the experiment.
This approach can be paired with measures of attention, learning, emotion, and decision-making. Researchers examine whether variation in regional perfusion corresponds to differences in performance or other behavioral results. Such comparisons provide a way to study how the delivery of neural resources relates to behavior across tasks, rather than treating brain circulation and behavioral measurements as separate sources of information.
The method is useful for investigating neurological disorders, evaluating treatment effects, and studying individual differences in brain function. In behavioral research, perfusion measures can add physiological context to observed differences in cognition or emotion-related performance. Comparing maps across individuals, conditions, or treatment settings can help identify how circulation-related brain changes accompany behavioral outcomes.