Serum Isolation Protocol
Serum Isolation Protocol - Related Videos
06:44
Isolation of Small Noncoding RNAs from Human Serum
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Cited by 13 •
2014
This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.
07:12
Neutrophil Isolation Protocol
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Cited by 173 •
2008
Neutrophils are among the first cells to arrive on the site of inflammatory immune response, and their functions and mechanisms have been studied extensively in vitro. We demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media.
08:59
Isolation and Culture of Microglia from Rat Brains using Serum-Free Media
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2025
This video demonstrates a method for isolating and culturing microglia from rat brains. It entails separating microglia from other neuronal and non-neuronal cells using plates coated with a monoclonal antibody specific to microglia.
08:43
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Cited by 9 •
2017
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
06:30
Protocol for Isolating the Mouse Circle of Willis
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Cited by 4 •
2016
We describe here a reproducible protocol for isolating the mouse circle of Willis.
FAQs
Consistent clotting time and temperature help standardize the sample before centrifugation. The blood must be allowed to complete clot formation under controlled conditions so the subsequent separation is comparable between samples. In biochemistry workflows, this consistency supports more reliable measurements and helps limit variation attributable to handling rather than to the specimen itself.
Centrifugation compacts the clot and blood cells into a pellet, leaving the clearer liquid above it as the recoverable fraction. The separation depends on keeping the phases distinct during processing. A well-defined pellet and undisturbed upper layer make it easier to collect serum while minimizing carryover of cellular or clot material.
Serum quality depends not only on forming the clot but also on separating the liquid fraction cleanly. Disturbing the compacted clot or cellular pellet can introduce unwanted material into the transferred sample. Careful separation therefore reduces cellular contamination and helps preserve serum for downstream measurement of biochemical targets.
After blood collection, allow the specimen to clot for a consistent period under controlled temperature conditions. Centrifuge the clotted sample to compact the clot and cells, then transfer the clear supernatant into a suitable receiving container without disturbing the pellet. These linked steps produce a cleaner sample for subsequent biochemical analysis.
Researchers use isolated serum when they need a liquid specimen for measuring proteins, enzymes, metabolites, antibodies, or other biomarkers. Its value extends across clinical testing, diagnostic work, and biochemical research, where consistent preparation supports comparison among samples. The appropriate use depends on the target measurement and the need to minimize cellular contamination during preparation.
In biochemistry, the separated sample provides access to several classes of measurable molecules without the compacted cellular and clot components. This makes it useful for examining protein and enzyme measurements, metabolite profiles, antibody-related analyses, and biomarker signals. The protocol therefore connects a physical separation step with analytical studies of biological composition.