Continuous Flow Electroporation
Continuous Flow Electroporation - Related Videos
10:34
The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
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Cited by 4 •
2022
This protocol describes the microfabrication techniques required to build a lab-on-a-chip, microfluidic electroporation device. The experimental setup performs controlled, single-cell-level transfections in a continuous flow and can be extended to higher throughputs with population-based control. An analysis is provided showcasing the ability to electrically monitor the degree of cell membrane permeabilization in real-time.
11:57
Electroporation of Mycobacteria
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Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
08:29
The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter
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Cited by 2 •
2014
This 3D microfluidic printing technology prints arrays of cells onto submerged surfaces. We describe how arrays of cells are delivered microfluidically in 3D flow cells onto submerged surfaces. By printing onto submerged surfaces, cell microarrays were produced that allow for drug screening and cytotoxicity assessment in a multitude of areas.
08:05
Murine In Utero Electroporation
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2023
In utero electroporation is an important technique for studying the molecular mechanisms that guide the proliferation, differentiation, migration, and maturation of cells during neural development. Electroporation enables the rapid and targeted delivery of material into cells by utilizing electrical pulses to create transient pores in cell membranes. Although electroporation has traditionally been used in in vitro studies, scientific advancements have now broadened its utilization to intact...
FAQs
As cells pass between electrodes, a brief electric pulse temporarily alters the cell membrane and creates pores. These openings permit DNA, RNA, proteins, or other biological cargo to cross into the cell. After the pulse, the membrane reseals, allowing the technique to combine transient permeability with continued cell processing.
Controlled pulses determine when cells experience membrane permeabilization as they move through the fluidic system. Their brief, regulated application supports cargo entry while allowing membrane resealing afterward. This timing is central to processing cells continuously rather than exposing them to an uncontrolled electrical treatment throughout the entire workflow.
The continuous-flow format processes cells as they travel through a fluidic pathway, whereas batch handling treats a collected population as a discrete group. This distinction can reduce reliance on repeated batch operations and support faster, more scalable processing. It also makes the approach compatible with integration into microfluidic platforms.
The method can introduce several classes of biological material, including DNA, RNA, proteins, and other cargo. That range makes it relevant to experiments requiring genetic material, gene-editing components, or protein delivery. The appropriate cargo depends on the intended cell-engineering or biological research objective.
A typical workflow moves cells and their selected cargo through a fluidic system, directs them between electrodes, and applies brief controlled electric pulses during passage. The temporary membrane pores allow entry, after which the membrane reseals as cells continue through the system. This arrangement supports an integrated, continuous processing sequence.
Researchers may choose this format when they need rapid processing, scalable handling, or consistent delivery across large cell populations. It is also useful when the transfection step must connect with a microfluidic workflow. These features make the method relevant to studies and development programs that cannot rely exclusively on discrete batch operations.
In bioengineering, the technique supports gene editing, cell-based therapies, vaccine development, and research requiring delivery of biological materials to many cells. Its continuous format can help connect molecular delivery with larger-scale cell processing. The method therefore serves both exploratory laboratory studies and workflows focused on scalable biological production.