Electrospun Scaffolds
Electrospun Scaffolds - Related Videos
09:32
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Cited by 5 •
2015
Here, we present a protocol to fabricate electrospun nanofiber scaffolds with gradated organization of fibers and explore their applications in regulating cell morphology/orientation. Gradients with regard to physical and chemical properties of the nanofiber scaffolds offer a wide variety of applications in the biomedical field.
06:14
Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
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Cited by 6 •
2019
This article demonstrates the technique of expanding a traditional, two-dimension (2D) electrospun nanofiber mat into a three-dimension (3D) scaffold through the depressurization of subcritical CO2 fluid. These augmented scaffolds are 3D, closely mimic cellular nanotopographic cues, and preserve the functions of biologic molecules encapsulated within the nanofibers.
16:03
The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
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Cited by 33 •
2011
Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.
08:03
Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells
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Cited by 9 •
2014
Synthesis and fabrication of electrospun long fibers spanning a larger deposit area via a newly designed collector from a novel biodegradable polymer named poly(glycerol-dodecanoate) (PGD) was reported. The fibers were able to support the growth of cells derived from mouse pluripotent stem cells.
15:52
Postproduction Processing of Electrospun Fibres for Tissue Engineering
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Cited by 30 •
2012
Electrospun scaffolds can be processed post production for tissue engineering applications. Here we describe methods for spinning complex scaffolds (by consecutive spinning), for making thicker scaffolds (by multi-layering using heat or vapour annealing), for achieving sterility (aseptic production or sterilisation post production) and for achieving appropriate biomechanical properties.
FAQs
Fiber diameter and alignment are adjusted through electrospinning conditions. Changes in processing conditions alter how the charged polymer jet travels toward the grounded collector, influencing whether fibers form with nanoscale-to-microscale dimensions and whether they display directional organization. These design variables let investigators tailor scaffold architecture for different tissue-engineering studies rather than relying on one fixed structure.
Solvent evaporation or cooling transforms the traveling polymer jet into a stable fibrous structure before collection. The relevant process depends on whether the starting material is a polymer solution or melt. This transition is essential because it determines when the material solidifies and helps produce continuous fibers suitable for constructing a scaffold with controlled dimensions and organization.
High surface area and tunable porosity give electrospun scaffolds structural features that can support cell attachment, growth, and tissue organization. Their extracellular-matrix-like architecture also provides a biomaterial framework for presenting other materials within the scaffold. Together, these properties make the structures useful as customizable substrates in bioengineering research rather than as passive bulk materials.
Production begins by placing a polymer solution or melt in a needle-based setup and applying a high-voltage electric field between the needle and a grounded collector. The field draws out a charged jet, while solvent evaporation or cooling solidifies it into fibers. Researchers can then use processing conditions to adjust fiber diameter and alignment for the intended scaffold design.
Electrospun scaffolds support research on skin, nerve, vascular, and bone tissue engineering. Their adjustable fiber dimensions, alignment, porosity, and matrix-like organization allow the scaffold structure to be matched to different tissue studies. This broad application range reflects the ability to create distinct biomaterial environments while retaining a fibrous architecture suitable for investigating cell growth and tissue organization.
The fibrous scaffold can be designed to support controlled presentation of biomaterials and potential therapeutic agents within a tissue-engineering setting. Its tunable architecture provides a configurable substrate rather than a single uniform surface, allowing researchers to investigate how scaffold structure and incorporated components relate to cell attachment, growth, or tissue organization. The specific presentation depends on the scaffold design.