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The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds

DOI:
10.3791/2389-v

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16:03 min

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February 15, 2011

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, , , , , , ,
1Department of Biomedical Engineering,University of Michigan, 2State Key Laboratory of Bioelectronics,Southeast University, 3Department of Neurology,University of Michigan , 4Geriatric Research, Education and Clinical Center,Veterans Affairs Ann Arbor Health System

Chapters

  • 00:05Title
  • 01:43Preparation of Electrospun Fiber Substrates
  • 04:40Primary Cell Culture of Rat Embryonic Neuron
  • 06:53Motor Neuron (MN) Processing
  • 09:29Sensory Neuron (SN) Processing
  • 10:56Plating and Culture
  • 12:41Representative Results
  • 14:40Conclusion

Summary

Automatic Translation

Automatic Translation

Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.

Tags

CulturePrimary Motor NeuronsSensory NeuronsDefined MediaElectrospun Nanofiber ScaffoldsElectrospinning TechniqueAlignmentRandom FibersBiodegradable PolymersPoly-L-lactic Acid (PLLA)Nerve RegenerationPrimary Cell Culture SystemBiomaterialsDorsal Root Ganglia ExplantsDissociated Neurons

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