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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
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Genetics
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JoVE Journal Genetics
Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

DOI:
10.3791/58918-v

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06:14 min

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January 07, 2019

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, , ,
1Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:43Prepare the Solutions for Standard Electrospinning and Set Up the Electrospinning Apparatus
  • 01:41Electrospinning
  • 02:21Generation of PCL Nanofiber Mats with Arrayed Holes
  • 02:41Expansion of 2D Nanofiber Mats with/without Arrayed Holes via Subcritical CO2 Liquid
  • 03:38Results: Visualization and In vivo Responses of PCL Nanofiber Scaffolds With and Without Expansion via Subcritical CO2
  • 05:25Conclusion

Summary

Automatic Translation

Automatic Translation

This article demonstrates the technique of expanding a traditional, two-dimension (2D) electrospun nanofiber mat into a three-dimension (3D) scaffold through the depressurization of subcritical CO2 fluid. These augmented scaffolds are 3D, closely mimic cellular nanotopographic cues, and preserve the functions of biologic molecules encapsulated within the nanofibers.

Tags

Keywords: Electrospun Nanofiber Mats2D To 3D TransformationPCL NanofibersSubcritical CO2 FluidDepressurizationElectrospinningScaffold Fabrication

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