Environmental Chamber Incubation
Environmental Chamber Incubation - Related Videos
09:46
Production and Measurement of Organic Particulate Matter in the Harvard Environmental Chamber
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Cited by 4 •
2018
This paper describes operation procedures for the Harvard Environmental Chamber (HEC) and related instrumentation for measuring gaseous and particle species. The environmental chamber is used to produce and study secondary organic species produced from the organic precursors, especially related to atmospheric organic particulate matter.
12:44
A General Method for Evaluating Incubation of Sucrose Craving in Rats
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Cited by 25 •
2011
Responding for food or drug-paired cues increases over the course of a period of abstinence and this may relate to an increased susceptibility to relapse behaviors. Here we detail a procedure for evaluating this "incubation of craving" in rats that have self-administered sucrose.
08:39
Simulating Temperature in a Soil Incubation Experiment
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Cited by 1 •
2022
Laboratory soil warming experiments usually employ two or more constant temperatures in multiple chambers. By presenting a sophisticated environmental chamber, we provide an accurate temperature control method to imitate the magnitude and amplitude of in situ soil temperature and improve the experimental design of soil incubation studies.
08:42
In Situ Gas Analysis and Fire Characterization of Lithium-Ion Cells During Thermal Runaway Using an Environmental Chamber
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Cited by 3 •
2023
Here, we describe a test procedure developed to characterize thermal runaway and fires in lithium-ion cells through in situ measurements of various parameters in an environmental chamber.
07:50
Construction of a Low-cost Mobile Incubator for Field and Laboratory Use
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Cited by 18 •
2019
This paper describes a method for building an adaptable, low-cost and transportable incubator for microbial testing of drinking water. Our design is based on widely available materials and can operate under a range of field conditions, while still offering the advantages of higher-end laboratory-based models.
FAQs
Continuous sensing and feedback control keep chamber conditions near their defined targets. Sensors track temperature, humidity, atmospheric composition, and light, while the control system directs heating, cooling, humidification, gas regulation, or lighting changes as needed. This coordinated response limits environmental drift, allowing observed biological changes to be associated more confidently with the experimental conditions.
Controlling temperature, humidity, atmospheric composition, and light allows researchers to hold some environmental factors steady while examining others. The same chamber can therefore support physiologically relevant conditions or deliberately altered ones. This flexibility is important when the goal is to evaluate how a biological sample grows, develops, responds to stress, or performs under a defined environment.
Enclosure and active regulation reduce fluctuations that could otherwise affect biological samples independently of the intended experiment. More stable conditions make repeated runs easier to compare and help researchers attribute differences in growth, development, stress response, or bioprocess performance to the tested environment. The main outcome is greater experimental consistency across investigations.
Before a run, researchers establish the desired temperature, humidity, atmospheric composition, and light conditions, then place the biological sample in the enclosed system. The chamber’s heating, cooling, humidification, gas-regulation, and lighting functions maintain those targets. This setup lets investigators compare growth or performance under specified environments rather than relying on uncontrolled surroundings.
Systematic changes reveal how biological systems respond to specific physical or atmospheric conditions. Researchers can examine effects on cells, tissues, microorganisms, or engineered biological systems while keeping the surrounding environment defined. Comparing responses across conditions can clarify patterns in development, stress responses, growth, or bioprocess performance, depending on the system being studied.
The method suits studies requiring controlled growth or evaluation of cells, tissues, microorganisms, and engineered biological systems. Applications may focus on development, stress responses, or bioprocess performance, especially when environmental variability would complicate interpretation. It also helps test whether a system maintains consistent behavior under physiologically relevant or deliberately altered conditions.