Fibroblast Explants
Fibroblast Explants - Related Videos
06:19
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
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Cited by 163 •
2013
The fibroblast explant culture protocol from human skin punch biopsies is a technically robust and simple way to derive skin cells within 4-8 weeks for banking of about 15-20 million cells at a low passage number.
06:04
Murine Dermal Fibroblast Isolation by FACS
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Cited by 28 •
2016
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
06:25
Explant Culture for Developmental Studies
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2023
Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo approach allows researchers to manipulate and observe developing tissues in ways that are not possible in vivo. Once established, explant culture is frequently used to understand the role of genes and signaling molecules in organogenesis. This video will first introduce the basic principles of explant culture and demonstrate a protocol to...
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
FAQs
Attachment is an early functional step: fibroblasts establish contact with the culture surface before spreading outward from the tissue fragment. Cells then migrate away from the explant and may proliferate as the culture provides nutrients and appropriate extracellular matrix cues. Observing these stages helps bioengineers distinguish changes in adhesion, movement, and population expansion.
Fibroblast behavior reflects several interacting inputs rather than a single culture condition. Nutrient availability supports cellular activity, while extracellular matrix cues influence attachment and outward movement. Mechanical and biochemical signals can further modify how cells respond and proliferate. Controlling or comparing these inputs allows researchers to examine how engineered environments affect repair-related cell behavior.
An explant retains a physical tissue fragment and preserves cell-matrix interactions that are difficult to capture when cells are studied only as a suspension. This context allows attachment, migration, and matrix-related behavior to be examined together. For bioengineering studies, the added structural context can make the model useful for evaluating how cells respond to biomaterials or scaffolds.
A basic workflow begins with preparing a small tissue fragment or isolated fibroblast-containing sample, placing it under controlled culture conditions, and allowing cells to attach to the culture surface. Researchers then observe outward migration and proliferation as the culture develops. The setup should provide nutrients and relevant extracellular matrix cues so cell behavior can be assessed consistently.
Researchers can evaluate whether cells attach effectively, how far they migrate from the explant, and whether the population expands over time. The model also supports examination of matrix deposition, a process relevant to tissue remodeling and wound repair. Together, these outcomes provide several readouts for comparing cellular responses to different culture environments or engineered materials.
In bioengineering, fibroblast explants can serve as practical test systems for biomaterials, engineered scaffolds, and regenerative strategies. Researchers can examine how these designs influence cell adhesion, matrix deposition, wound repair, and tissue remodeling while retaining cell-matrix interactions. This makes the approach useful when a study needs more biological context than a simplified cell suspension can provide.