Follicle Expansion Analysis
Follicle Expansion Analysis - Related Videos
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
10:31
Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
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Cited by 2 •
2025
This protocol describes a novel three-dimensional (3-D) culture model using a tyramine-linked hyaluronan hydrogel to encapsulate and culture preantral follicles from the mouse ovary. We also detail two approaches to ovarian follicle cryopreservation by vitrification.
12:37
A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
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Cited by 80 •
2011
A new method for ovarian follicle encapsulation in a 3D fibrin-alginate interpenetrating network is described. This system combines structural support with proteolytic degradation to support the development of immature follicles to produce mature oocytes. This method may be applied to culture cell aggregates to maintain cell-cell contacts without limiting expansion.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
13:58
Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
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Cited by 26 •
2014
Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.
FAQs
Diameter, area, and volume capture different aspects of follicle enlargement and should be selected according to the analysis goal. Diameter provides a direct size measure, whereas area summarizes the follicle’s two-dimensional extent and volume represents a three-dimensional estimate. Tracking one or more of these metrics over time creates a quantitative basis for comparing growth across culture conditions.
Size measurements become more informative when paired with morphology and integrity. A follicle may change in dimensions while also showing structural changes that affect how its development is interpreted. Recording shape-related appearance and preservation of tissue organization alongside expansion therefore helps distinguish simple dimensional change from broader changes in follicle condition. This combined readout is especially relevant in engineered reproductive tissues.
Culture conditions are central variables in interpreting expansion results because they can influence follicle maintenance and maturation. In bioengineering studies, researchers may examine how biomaterials, scaffold design, biochemical signals, or the surrounding culture environment affect the measured trajectory. Comparing these factors with the same size and structural readouts helps identify which experimental conditions better support follicle development.
Microscopy and time-lapse imaging both support measurements of follicle change over time, but they provide different ways to observe that progression. A time-lapse approach records development as a sequence, while microscopy can supply measurements at selected observations. In either case, consistent assessment of size, morphology, and integrity helps ensure that imaging results support comparisons between culture conditions.
A practical workflow starts by maintaining follicles under controlled culture conditions, acquiring images over time, and measuring diameter, area, or volume from those observations. Researchers then examine morphology and integrity before comparing the resulting growth patterns among experimental groups. This sequence connects image-based measurements with evidence about how effectively each culture condition supports follicle maintenance and maturation.
In bioengineering, follicle expansion analysis provides a measurable way to compare three-dimensional culture systems that use different biomaterials or scaffold designs. Growth measurements can be evaluated alongside morphology and integrity to determine whether a system supports both enlargement and preservation of follicle structure. These comparisons guide refinement of engineered reproductive tissue models and their culture environments.
The resulting measurements can support optimization of engineered reproductive tissues by showing how follicles respond to culture conditions, biomaterials, scaffold architectures, and biochemical signals. This information helps researchers compare experimental systems and identify approaches associated with follicle maintenance and maturation. The broader relevance includes developing strategies for fertility preservation and regenerative medicine.