Force Curve Acquisition
Force Curve Acquisition - Related Videos
01:04
Hydrostatic Pressure Force on a Curved Surface
0 Views •
2025
Hydrostatic pressure on curved surfaces is a fundamental concept in fluid mechanics with broad applications in the civil engineering field. When fluid is in contact with a curved surface, as in a reservoir, dam, or storage tank, it exerts pressure that varies in magnitude and direction along the curved surface. To assess the total hydrostatic force exerted by the fluid on a curved structure, engineers typically isolate the fluid volume adjacent to the surface and analyze the forces acting on...
02:44
Heating and Cooling Curves
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2020
When a substance—isolated from its environment—is subjected to heat changes, corresponding changes in temperature and phase of the substance is observed; this is graphically represented by heating and cooling curves. For instance, the addition of heat raises the temperature of a solid; the amount of heat absorbed depends on the heat capacity of the solid (q = mcsolidΔT). According to thermochemistry, the relation between the amount of heat absorbed or released by a substance, q, and its...
07:43
Calibration Curves
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Calibration curves are used to understand the instrumental response to an analyte and predict the concentration in an unknown sample. Generally, a set of standard samples are made at various concentrations with a range than includes the unknown of interest and the instrumental response at each concentration is recorded. For more accuracy and to understand the error, the response at each concentration can be repeated so an error...
10:15
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Cited by 34 •
2015
We describe a protocol for preparation of supported lipid bilayers and its characterization using atomic force microscopy and force spectroscopy.
07:46
Data Acquisition Protocol for Determining Embedded Sensitivity Functions
0 Views •
2016
The data acquisition procedure for determining embedded sensitivity functions is described. Data is acquired and representative results are shown for a residential scale wind turbine blade.
FAQs
During acquisition, an atomic force microscopy probe moves toward the sample and then withdraws. The laser records cantilever deflection throughout both directions, creating a force–distance curve rather than a single measurement. Changes during approach reflect contact and deformation, while the withdrawal portion captures adhesion and the point at which the probe separates from the surface.
These features describe different stages of the probe–sample interaction. Contact begins when the probe engages the sample, and subsequent displacement reflects deformation under loading. Adhesion appears during withdrawal when the sample and probe remain coupled, while separation marks release. Together, these regions allow mechanical behavior to be interpreted across the loading cycle.
Mathematical models are applied to the measured force–distance relationship to connect probe response with sample deformation. Depending on the curve features and the selected model, analysis can estimate stiffness, elasticity, or adhesion forces. These calculated properties provide quantitative comparisons among samples instead of relying only on qualitative observations of surface interaction.
A typical acquisition positions the probe over the sample, drives the probe toward the surface under controlled mechanical motion, records cantilever deflection with a laser, and then retracts the probe while continuing measurement. The resulting approach and withdrawal data are assembled into a force–distance curve for subsequent analysis of contact, deformation, adhesion, and separation.
Researchers can compare estimated stiffness, elasticity, and adhesion forces between different samples or regions. Such comparisons show how materials respond to the same type of controlled mechanical loading and can distinguish differences in mechanical behavior. In bioengineering, these measurements support comparisons involving cells, tissues, biomaterials, and engineered surfaces.
The technique links microscale and nanoscale mechanical behavior with biological structure and function. Measurements can be used to compare cells and tissues, examine biomaterials, and evaluate engineered surfaces. These mechanical comparisons may also help investigate differences associated with disease state or assess how a material's behavior relates to device performance.