Ht 29 Cells
Ht 29 Cells - Related Videos
14:26
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
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Cited by 59 •
2016
This paper presents a sensitive method called Circle-Seq for purifying extrachromosomal circular DNA (eccDNA). The method encompasses column purification, removal of remaining linear chromosomal DNA, rolling-circle amplification and high-throughput sequencing. Circle-Seq is applicable to genome-scale screening of eukaryotic eccDNA and studying genome instability and copy-number variation.
04:22
Intracellular Replication Assay for Shigella in Human Colonic Epithelial Cells
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2026
Source: Poore, K., et al. Epithelial Cell Infection Analyses with Shigella. J. Vis. Exp. (2024)This video demonstrates an in vitro assay to assess Shigella invasion and intracellular replication in human colonic epithelial monolayers. It highlights steps for infection, antibiotic selection, host cell lysis, and recovery of intracellular bacteria for downstream analysis.
16:04
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
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Cited by 66 •
2009
This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
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Cited by 56 •
2010
The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
02:36
Bacteriophage Synthesis Using a Cell-Free Transcription-Translation System
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2026
Source: Rustad, M., et al. Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System. J. Vis. Exp. (2017).This video demonstrates the synthesis of bacteriophages using a cell-free transcription–translation (TXTL) system. A master mix is prepared using a bacterial crude extract, an energy mix, an amino acid mix, an inhibitor for a DNA-degrading enzyme, and bacteriophage DNA, then incubated. The in vitro molecular machinery enables viral protein expression and DNA...
FAQs
HT-29 cells grow as adherent epithelial cells, so experiments depend on maintaining a surface-associated population under appropriate culture conditions. Their human colorectal origin makes epithelial responses relevant to intestinal and tumor studies, while their reproducible proliferation supports controlled comparisons. In selected sublines or differentiation protocols, investigators can also examine mucin production and more specialized intestinal phenotypes.
Selected sublines and differentiation protocols shift the experimental phenotype beyond simple proliferative growth. Under those conditions, HT-29 cells may produce mucin and display more specialized intestinal characteristics, creating a way to investigate epithelial maturation in a controlled in vitro setting. This distinction matters because findings from undifferentiated cultures should not automatically represent specialized intestinal cells.
HT-29 cells retain cancer-associated characteristics, meaning their behavior may reflect tumor-associated epithelium rather than healthy intestinal tissue. Consequently, findings can support studies of colorectal cancer biology or tumor-associated epithelial behavior, but they should not substitute for conclusions about normal intestine. This limitation is especially important when interpreting responses observed in engineered tissue systems.
An HT-29 experiment generally begins by establishing an adherent culture under appropriate conditions, followed by expansion to obtain a reproducible epithelial population. Researchers can then apply a selected differentiation protocol or incorporate the cells into an engineered gut model, depending on the question. The resulting system may be evaluated for epithelial behavior, mucin-related features, transport, toxicity, or tissue-interface responses.
In bioengineering, HT-29 cells provide a human epithelial component for constructing and testing gut models, biomaterials, and tissue interfaces. Their use allows investigators to examine how an engineered environment affects intestinal epithelial or tumor-associated behavior under controlled conditions. Reproducible proliferation also helps researchers compare different material designs or model configurations across experiments.
HT-29 cells can support in vitro evaluation of drug transport and toxicity by providing a human colorectal epithelial context. Within engineered gut systems, investigators may examine how compounds interact with an epithelial model, while cancer-associated properties add relevance to tumor-related questions. Interpretation should distinguish epithelial transport or toxicity observations from conclusions about healthy intestinal physiology.