Mouse Ivd
Mouse Ivd - Related Videos
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Cited by 22 •
2017
Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.
07:04
A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
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Cited by 5 •
2017
Mice are widely used to study gestational biology. However, pregnancy termination is required for such studies which precludes longitudinal investigations and necessitates the use of large numbers of animals. Therefore, we describe a non-invasive technique of high-frequency ultrasonography for early detection and monitoring of post-implantation events in the pregnant mouse.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
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Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
04:27
Generating a Pro-Inflammatory Organ Culture Model to Simulate an Early Intervertebral Disc Disease
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2025
This video demonstrates a technique for culturing bovine intervertebral discs in bioreactors to develop a three-dimensional organ culture model. Specific nutrient-deprived media and degenerative pressure conditions are applied to the discs, mimicking the pro-inflammatory and degenerative microenvironment observed during early-stage intervertebral disc disease. The disc heights are measured post-treatment to analyze the effects of nutrient deprivation and degenerative loading.
FAQs
The three tissue regions contribute complementary mechanical functions. The gelatinous nucleus pulposus supports fluid-mediated load transfer, while the collagen-rich annulus fibrosus contains and organizes the surrounding structure. Vertebral endplates border the disc and complete the load-bearing arrangement. Studying these interactions helps bioengineers connect extracellular-matrix organization with changes in disc mechanics.
Matrix organization determines how disc tissues distribute and respond to mechanical forces. Changes in collagen-rich structure or the matrix surrounding disc cells can alter compression behavior and fluid-mediated load transfer. In Mouse IVD studies, examining these relationships links cellular or material changes to biomechanical outcomes, providing a mechanistic basis for evaluating degeneration and repair strategies.
Genetic approaches can identify how altered biological programs affect disc development, maintenance, or degeneration, while mechanical approaches test how loading conditions influence tissue responses. Combining these perspectives separates biologically driven changes from load-related effects. This comparison is valuable because disc pathology can be examined as an interaction between cellular regulation, tissue structure, and mechanical demands.
These models support complementary genetic, mechanical, and imaging investigations. Genetic analysis can relate particular biological changes to disc phenotypes, mechanical assessment can examine load-related behavior, and imaging can document structural changes. Together, the resulting evidence connects tissue architecture, extracellular-matrix organization, and function, helping researchers evaluate how experimental interventions influence disc condition.
Researchers can use the model when they need to investigate disc development, degeneration, inflammation, or repair within an experimentally accessible system. Its value increases when genetic, mechanical, and imaging methods must be integrated with tissue-engineering approaches. Such studies can reveal how cellular and material changes affect disc behavior before informing biomaterial or regenerative strategy design.
A Mouse IVD model provides a setting for examining whether engineered materials or repair approaches preserve tissue organization and mechanical function. Researchers can compare structural, cellular, and load-related changes during degeneration or repair. These outcomes help determine how material properties and biological responses interact, supporting the refinement of regenerative strategies and preclinical interventions for spinal disorders.