Lung Lavage
Lung Lavage - Related Videos
07:03
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
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Cited by 155 •
2017
The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.
02:17
Bronchoalveolar Lavage Fluid Collection from a Mouse Model Infected with Non-typeable Haemophilus influenzae
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2025
Source: Venuprasad, K., et al. Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation. J. Vis. Exp. (2016)This video demonstrates the collection and processing of bronchoalveolar lavage fluid from a euthanized mouse infected with non-typeable Haemophilus influenzae for downstream analysis.
07:20
Lavage-induced Surfactant Depletion in Pigs As a Model of the Acute Respiratory Distress Syndrome (ARDS)
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Cited by 21 •
2016
Repeated pulmonary lavages in anesthetized pigs induce lung injury resembling major aspects of human acute respiratory distress syndrome (ARDS). For this purpose the lungs are repeatedly lavaged with 0.9% saline at 37 °C. The goal of the protocol is a reproducible mitigation of gas exchange and hemodynamics for research in ARDS.
08:27
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
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Cited by 57 •
2018
Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.
03:37
Visualization of Extracellular Traps in Macrophages from Human Bronchoalveolar Lavage Fluids
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2025
This video demonstrates the immunolabeling of macrophage extracellular traps, METs, in bronchoalveolar macrophages from bronchoalveolar lavage fluid, and their visualization using a confocal microscope.
FAQs
The recovered saline carries material that has mixed with it inside the sampled airway or lung compartment, including mucus, cells, proteins, and accumulated particles. This makes the lavage fluid a composite sample rather than a direct tissue specimen. Researchers can examine its contents to characterize local pulmonary conditions and assess how biological material changes during disease or treatment.
Bronchoalveolar lavage targets the lower airways and is primarily valuable for collecting biological samples. Whole-lung lavage addresses a broader lung compartment and can provide a clearance function by removing abnormal accumulated material. This distinction determines whether the procedure is being used mainly for localized sampling or for reducing material distributed through the lung, such as in pulmonary alveolar proteinosis.
Recovered fluid contains biological material from the sampled respiratory environment, allowing investigators to examine inflammation, infection, or other disease-associated changes without relying only on an engineered model or therapy outcome. Its composition can also help evaluate how pulmonary systems respond to interventions, making lavage useful for connecting local lung biology with bioengineering experiments.
A typical sampling workflow places a catheter or bronchoscope into the relevant airway, delivers sterile saline, allows the fluid to mix with material in the target compartment, and withdraws the mixture for analysis. The sequence links the selected sampling route with the resulting specimen, so the recovered fluid reflects the compartment reached by the instrument.
Researchers may choose bronchoalveolar lavage when they need lower-airway samples to study pulmonary inflammation, infection, respiratory disease, or responses to drug delivery. The approach is especially useful when an experiment requires biological material from the lung environment rather than only an external measurement. It can therefore support assessment of disease processes and engineered interventions in a localized respiratory setting.
Lavage provides recovered fluid that can be examined after an engineered therapy or within studies using lung models. Comparing the collected material across experimental conditions can help evaluate pulmonary responses and treatment-related effects. In this way, the procedure supplies biological evidence for judging whether an engineered system captures relevant features of lung inflammation, infection, drug delivery, or respiratory disease.