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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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Bioengineering
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JoVE Journal Bioengineering
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

DOI:
10.3791/56105-v

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13:42 min

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September 19, 2017

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, , ,
1Department of Microsystems Engineering,University of Freiburg, 2Freiburg Materials Research Center,University of Freiburg, 3Department of Biosystems Science and Engineering,ETH Zurich

Chapters

  • 00:05Title
  • 01:11Fabrication of the Microfluidic Biosensor Using Dry Film Photoresist (DFR) Technology
  • 07:29On-chip Assay Immobilization Procedure
  • 09:34Amperometric Signal Detection Using the Stop-flow Technique
  • 11:12Results: Working Principle of the Biosensor and the Signal Readout
  • 12:26Conclusion

Summary

Automatic Translation

Automatic Translation

A microfluidic biosensor platform was designed and fabricated using low-cost dry film photoresist technology for the rapid and sensitive quantification of various analytes. This single-use system allows for the electrochemical readout of on-chip-immobilized enzyme-linked assays by means of the stop-flow technique.

Tags

AI-based BiosensorElectrochemical BiosensorDry Film PhotoresistMicrofluidic PlatformPoint-of-care TestingAnalyte QuantificationLow-cost FabricationVersatile Application

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