Microwell Imaging
Microwell Imaging - Related Videos
02:25
Photodegradable Hydrogel-Based Isolation of Bacterial Colonies in Microwell Arrays
0 Views •
2025
Source: Fattahi, N., et al. Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation. J. Vis. Exp. (2021)This video demonstrates the use of photodegradable hydrogels to isolate bacterial colonies within silicon microwell arrays. Bacterial cells are first loaded into microwells, encapsulated in a crosslinked hydrogel, and cultured into distinct colonies. Upon exposure to ultraviolet light, targeted degradation of the hydrogel enables selective extraction of individual...
10:44
Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
0 Views •
Cited by 36 •
2013
We introduce a protocol for the generation of large numbers (thousands to hundreds of thousands) of uniform size- and composition-controlled tumor spheroids, using commercially available microwell plates.
06:28
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
0 Views •
Cited by 11 •
2016
Conventional methods to initiate suspension aggregate based cardiac differentiation of human pluripotent stems cells (hPSCs) are plagued with culture heterogeneity with respect to aggregate size and shape. Here, we describe a robust method for cardiac differentiation employing microwells to generate size-controlled hPSC aggregates cultured under cardiac-promoting conditions.
09:24
Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
0 Views •
Cited by 12 •
2017
The development of microbial communities depends on a combination of factors, including environmental architecture, member abundance, traits, and interactions. This protocol describes a synthetic, microfabricated environment for the simultaneous tracking of thousands of communities contained in femtoliter wells, where key factors such as niche size and confinement can be approximated.
11:42
A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries
0 Views •
Cited by 8 •
2018
This manuscript introduces a robust method of fabricating concave microwells without the need for complex high-cost facilities. Using magnetic force, steel beads, and a through-hole array, several hundred microwells were formed in a 3 cm x 3 cm polydimethylsiloxane (PDMS) substrate.
FAQs
By confining a biological unit within a defined microenvironment, each well provides a spatially organized region for observation. This arrangement helps isolate or organize neighboring samples and makes measurements easier to associate with a particular cell aggregate, spheroid, organoid, particle, or engineered tissue. Imaging across the array then supports consistent comparison among many localized samples.
Bright-field imaging can document visible morphology and structural organization, while fluorescence imaging records fluorescent signals when those signals are relevant to the study. Other optical approaches may also be used. Across these modes, images can support analysis of morphology, growth, behavior, interactions, viability, structure, and function.
An array allows multiple biological units to be observed under the same general imaging framework rather than examined as isolated examples. Because each microwell supplies a defined location, image-based comparisons can be organized across samples. This parallel format is particularly useful for characterizing cell aggregates, spheroids, organoids, biomaterials, and microfabricated systems.
After optical images are recorded, researchers examine measurable changes in the visual properties of the confined samples. Depending on the study, analysis can focus on morphology, growth, behavior, interactions, viability, structure, or function. Comparing these measurements across wells turns spatially organized images into data for characterizing engineered biological systems.
Spheroids and organoids can be evaluated as organized biological structures within defined microwell locations. Imaging allows researchers to examine their morphology, growth, viability, and structural changes across many samples. These observations support characterization of engineered tissues and provide information relevant to tissue engineering, disease modeling, and optimization of cellular platforms.
In bioengineering, the method connects microfabricated sample organization with image-based measurement. Researchers can assess engineered cellular platforms, biomaterials, aggregates, spheroids, organoids, and other systems for changes in structure, viability, and function. The resulting comparisons can inform tissue engineering strategies, disease models, and efforts to optimize engineered biological systems.