/
/
Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:50Bacterial Seeding
- 02:31Sealing the Wells with an Agarose Coated Coverslip and Imaging
- 04:53Bacterial Growth Analysis
- 07:10Results: Growth of P. aeruginosa Mutants
- 08:28Conclusion
The development of microbial communities depends on a combination of factors, including environmental architecture, member abundance, traits, and interactions. This protocol describes a synthetic, microfabricated environment for the simultaneous tracking of thousands of communities contained in femtoliter wells, where key factors such as niche size and confinement can be approximated.
