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Human Dupuytren’s Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
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Human Dupuytren’s Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions

Human Dupuytren’s Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions

DOI:
10.3791/52534-v

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08:59 min

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April 18, 2015

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, , , , ,
1Department of Molecular Cell Biology, Cancer Genomics Centre and Centre for Biomedical Genetics,Leiden University Medical Center, 2Department of Orthopedic Surgery,Academic Medical Center, 3Department of Plastic, Reconstructive and Hand Surgery,Academic Medical Center, 4Department of Orthopedic Surgery,Mayo Clinic, 5Department of Biochemistry and Molecular Biology,Mayo Clinic, 6Department of Dermatology,Leiden University Medical Center

Chapters

  • 00:05Title
  • 02:06Tissue Preparation and Ex Vivo Tissue Culture Setup
  • 05:27Combined Second Harmonic Generation (Collagen) and Two-photon Excited Fluorescence (Elastin) Imaging on Ex Vivo Tissue during Culture
  • 06:39Results: Effects on 3D Extracellular Matrix Composition upon Tissue Manipulation by Compound Treatments and Viral-mediated Gene Modulation
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

Dupuytren’s disease (DD) is a fibroproliferative disease of the palm of the hand. Here, we present a protocol to culture resection specimens from DD in a three-dimensional (3D) culture system. Such short-term culture system allows preservation of the 3D structure and molecular properties of the fibrotic tissue.

Tags

Keywords: Dupuytren’s ContractureOrgan FibrosisEx Vivo CultureMyofibroblastsExtracellular Matrix3D CultureProliferationIn Vivo Model

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