On-chip Valve
On-chip Valve - Related Videos
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
10:36
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
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Cited by 6 •
2012
We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.
02:45
Bacterial Growth on a Microfluidic Chip to Study Antibiotic Drug Resistance
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2025
Source: Liu, P. C., et al. Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection. J. Vis. Exp. (2016)This video demonstrates the use of a microfluidic chip to visualize and compare the growth and morphology of wild-type and trimethoprim-resistant E. coli strains under trimethoprim antibiotic stress.
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
08:55
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
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Cited by 16 •
2017
This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process.
FAQs
The actuation mechanism determines how a valve changes the state of a microfluidic channel. Pneumatic pressure, mechanical deformation, and material properties provide different ways to produce that change, allowing designers to match valve behavior to tasks such as controlling flow, isolating compartments, or coordinating fluid movement. This choice directly affects how the microfluidic system performs its intended operation.
Timing allows fluid operations to occur at precise locations and moments, while isolation keeps selected regions of a microfluidic system separated. Together, these functions support sequential processing, compartmentalization, and controlled exposure of fluids to one another. Their combination is especially valuable when an assay requires multiple stages or when different samples and reagents must remain distinct.
A single valve can regulate a local fluidic event, but coordinated valves can organize a larger workflow. By opening, closing, or isolating different parts of the system in sequence, valve networks support routing, metering, mixing, and compartmentalization. This coordination enables automated assays rather than requiring each fluid-handling step to be performed independently.
A typical workflow begins by identifying where fluid routing, metering, mixing, or isolation is required. Valves are then integrated at those locations and coordinated with the assay sequence so fluids move or remain separated at the appropriate times. The resulting configuration can automate the intended experiment while reducing manual handling within the microfluidic platform.
These valves are useful when a platform must manage several fluidic operations within a small device. In cell culture, they can support controlled fluid handling and compartmentalization. In chemical analysis and point-of-care diagnostics, they can coordinate routing, metering, mixing, and isolation, helping organize multistep assays in compact lab-on-a-chip systems.
Integrated valve control can reduce the volumes of samples and reagents needed for an experiment while improving the reproducibility of fluid-handling steps. Automation also supports more consistent execution of complex assays and can contribute to portable bioengineering platforms. These benefits make valve-based microfluidic systems useful for experiments that require repeatable, compact operation.