Peristaltic Bioreactor
Peristaltic Bioreactor - Related Videos
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
04:21
Controlled Infusion of Radiopharmaceutical Agent Using a Peristaltic Pump
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2025
Source: Léa Rubira1, Hélène Sturer1, Louise Combette1, Sandra Santini1, Stéphane Renaud1, Cyril Fersing1,21Nuclear medicine department, Institut régional du Cancer de Montpellier (ICM), Univ Montpellier, 2IBMM, Univ Montpellier, CNRS, ENSCMThis video demonstrates the safe, controlled infusion of a radiopharmaceutical using a peristaltic pump with a dual-line cassette system. The setup includes connecting saline and infusion lines, positioning the shielded vial, and initiating simultaneous...
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
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Cited by 8 •
2015
A novel reactor design, coined a high density bioreactor (HDBR), is presented for the cultivation and study of high density microbial communities. Here, the HDBR is successfully applied in a photobioreactor (PBR) configuration for the study of nitrogen metabolism by a mixed high density algal community.
09:46
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
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Cited by 12 •
2017
Chondrogenesis from stem cells requires fine tuning the culture conditions. Here, we present a magnetic approach for condensing cells, an essential step to initiate chondrogenesis. In addition, we show that dynamic maturation in a bioreactor applies mechanical stimulation to the cellular constructs and enhances cartilaginous extracellular matrix production.
FAQs
Flow generation depends on timed compression of flexible tubing at successive positions. As each roller or actuator closes and releases the tubing, the compressed region travels along the channel and displaces the culture medium forward. This arrangement creates a controllable moving flow pattern while keeping the drive components physically separated from the biological material.
The moving medium exposes cells or tissue constructs to fluid shear, meaning mechanical force generated by flow, while the compression sequence supplies repeated mechanical stimulation. Together, these cues help reproduce aspects of physiological loading in an engineered environment. Their value is especially relevant when researchers study how developing tissues respond to combined fluid and mechanical signals.
Because the rollers or actuators compress the tubing from outside, they do not directly contact the culture medium. This separation can support sterile operation by isolating the drive mechanism from the fluid pathway. At the same time, controlled circulation helps move medium through the system, improving mass transport around cells or tissue constructs.
A basic setup requires flexible tubing, a mechanism that applies rotating or sequential compression, culture medium, and the cells or tissue constructs being maintained. The tubing provides the fluid pathway, while the actuator sequence establishes movement through it. The biological material can therefore be cultured under flow and cyclic mechanical conditions without placing the drive mechanism in the medium.
Researchers may select this system for tissue engineering, three-dimensional cell culture, or experiments examining how engineered tissues respond to flow and mechanical cues. It is particularly relevant when a study needs more than static culture conditions, because the setup can provide fluid movement together with repeated mechanical stimulation while supporting controlled handling of the culture environment.
The system helps investigators examine tissue growth and maturation under dynamic culture conditions, including responses to fluid shear and cyclic loading. It can also support evaluation of how flow influences mass transport around three-dimensional constructs. In bioengineering, these observations provide a way to relate engineered-tissue behavior to controlled mechanical and fluid cues.