Retinal Laser Surgery

Retinal laser surgery is a minimally invasive treatment that uses focused light energy to repair or stabilize damaged retinal tissue and preserve vision. In laser photocoagulation, a precisely controlled beam is directed through the eye to create small, localized thermal effects that seal leaking blood vessels, reinforce retinal tears, or reduce abnormal tissue growth while limiting damage to surrounding structures. The procedure supports care for conditions such as diabetic retinopathy, retinal tears, and selected vascular disorders, while also illustrating bioengineering principles in optical focusing, energy delivery, and tissue response. Advances in imaging and laser control continue to improve treatment precision and safety.

Retinal Laser Surgery - Related Videos

Research

JoVE EoE - Neuropathology

Modeling Retinal Degeneration and Regeneration in Zebrafish Using a Focal Laser Injury

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2025

This video demonstrates a procedure to induce retinal degeneration in adult zebrafish using laser injury. The laser targets photoreceptor cells in the retina, triggering cell death and a degenerative process. This damage activates Müller glia (MG) cells, leading to their dedifferentiation into stem cell progenitors that aid in retinal regeneration.

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

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Cited by 13 •

2017

The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

Research

JoVE Journal - Medicine
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A Step by Step Protocol for Subretinal Surgery in Rabbits

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Cited by 30 •

2016

Retinal pigment epithelium (RPE) replacement strategies and gene-based therapy are considered for several retinal degenerative conditions. For clinical translation, large eye animal models are required to study surgical techniques applicable in patients. Here we present a rabbit model for subretinal surgery geared towards RPE transplantation, which is versatile and cost-efficient.

An “All-laser” Endothelial Transplant

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Cited by 5 •

2015

An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.

An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells

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Cited by 26 •

2011

This article provides a description of how to dissect and record from the isolated retinal preparation in mouse. In particular, we describe how to record light responses from a fluorescently labeled ganglion cell population and subsequently identify and analyze its morphology.

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