Rotating Wall Vessel
Rotating Wall Vessel - Related Videos
12:22
Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models
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Cited by 80 •
2012
A rotating cell culture system that allows epithelial cells to grow under physiological conditions resulting in 3-D cellular aggregate formation is described. The aggregates generated display in vivo-like characteristics not observed in conventional culture models and serve as a more accurate organotypic model system for a multitude of scientific investigations.
08:28
Monitoring the Wall Mechanics During Stent Deployment in a Vessel
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2012
Stent-induced arterial strain distributions are characterized using an optical surface strain measurement system. This visualization technique is used to gain insights into the impact of stent implantation on the host vessel.
01:20
Congo Red Staining to Visualize Amyloid Fibrils in Cerebral Vessel Walls
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2025
The tissue shows amyloid fibrils and abnormal protein aggregates in the cerebral vessel walls, which are key indicators of neurological disorders.
10:24
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
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Cited by 13 •
2015
To study the interaction of bacteria with the blood vessels under shear stress, a flow chamber and an in vivo mesenteric intravital microscopy model are described that allow to dissect the bacterial and host factors contributing to vascular adhesion.
05:56
Mental Rotation
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Visual mental imagery refers to the ability to conjure images in one’s mind’s eye. This allows people to process visual material above and beyond the constraints of a current point-of-view; for example, a person could imagine, using their mind’s eye, how something might look in a different color, or what it would look like if it were made from a different material or rotated and seen from a different perspective. Mental imagery...
FAQs
Continuous rotation keeps suspended cells, tissues, or engineered constructs in free fall relative to the vessel wall. This reduces sedimentation and limits direct mechanical forces associated with settling against a stationary surface. The resulting low-shear environment allows three-dimensional cultures to remain suspended while still receiving nutrient and gas exchange from the surrounding fluid.
Low shear limits potentially damaging mechanical forces that can affect delicate three-dimensional cultures. By reducing these forces, the system supports the formation and maintenance of tissue-like aggregates, organoids, and engineered constructs. This is especially relevant when researchers want to examine cell behavior or tissue development in structures that more closely resemble native tissue architecture than flat cultures.
Conventional monolayer culture grows cells on a surface, whereas the rotating system supports suspended three-dimensional organization. This difference can produce tissue-like aggregates, organoids, and other constructs with architecture that more closely reflects native tissue. Consequently, the vessel provides a complementary bioengineering model for studying cellular behavior, tissue development, and engineered materials under controlled laboratory conditions.
The fluid-filled vessel keeps culture contents suspended while maintaining contact with the surrounding medium during rotation. This arrangement supports nutrient and gas exchange without requiring the cells or constructs to remain attached to a flat surface. Maintaining exchange is important because it enables researchers to sustain three-dimensional cultures while examining their development and behavior in a controlled environment.
A basic workflow places cells, tissues, or engineered constructs in the vessel’s fluid environment and then maintains continuous rotation during culture. Researchers use the controlled system to keep the material suspended and reduce sedimentation. The resulting cultures can then be examined as three-dimensional aggregates, organoids, or engineered constructs for studies of development, behavior, disease, or biomaterial performance.
These systems support investigations of cell behavior, tissue development, disease processes, and biomaterial performance. They are useful when the research question depends on three-dimensional organization rather than only on responses from cells grown as a monolayer. In bioengineering, the approach also provides a controlled setting for evaluating tissue-like models and engineered constructs during culture.