Stem Cell Recruitment
Stem Cell Recruitment - Related Videos
11:30
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
0 Views •
Cited by 28 •
2015
The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
0 Views •
Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
04:28
Lung Tumor Cell Recruitment Assay
0 Views •
2019
This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
0 Views •
2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
20:38
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
0 Views •
Cited by 31 •
2007
FAQs
Directed migration depends on spatial differences in signaling rather than on a uniform signal. Injured or engineered tissues can create gradients of chemokines and growth factors, while extracellular matrix cues and cell-adhesion interactions help cells interpret the local environment. Together, these cues influence whether stem cells move toward the target and remain positioned there long enough to support repair or regeneration.
Extracellular matrix cues and adhesion interactions contribute to more than initial movement. They help establish how recruited cells interact with the target tissue and can support retention at the repair site. This distinction matters because attracting cells without maintaining their localization may not provide the sustained cellular presence needed for tissue support, integration, or functional recovery.
Outcome depends on the relationship between signal production, migration, and retention. Injury can supply endogenous cues, whereas an engineered construct can be designed to present chemokines, growth factors, matrix signals, or adhesion-related cues. The resulting local environment affects how effectively cells are directed to the target and whether recruitment contributes to repair of complex tissues.
Bioengineers use biomaterial scaffolds and tissue-engineered constructs as platforms for presenting recruitment signals at a damaged site. Therapeutic delivery systems provide another route for incorporating these cues. The design objective is to create a local environment that attracts endogenous stem cells and supports their localization, linking signal presentation with tissue integration and regenerative performance.
Recruiting endogenous stem cells can be valuable when a strategy aims to reduce reliance on transplanted cells. Instead of supplying all reparative cells directly, the engineered system is intended to draw available stem cells toward the damaged tissue. This approach may support repair, regeneration, or functional recovery while directing the cellular response to the specific target site.
Assessment can focus on whether recruited cells reach and remain at the intended tissue and whether their presence is associated with useful biological outcomes. Relevant goals include improved tissue integration, repair, regeneration, and functional recovery. In bioengineering studies, these outcomes connect the behavior of recruited cells with the performance of the scaffold, construct, or delivery system.