Urothelial Reconstruction
Urothelial Reconstruction - Related Videos
03:32
Patient-derived Tumor Xenografting: A Technique to Generate Experimental Mouse Model for Evaluating Urothelial Cell Carcinoma
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2023
This video describes the procedure to generate an experimental xenograft murine model, which can be used for studying urothelial cell carcinoma progression.
03:56
Delivery of DNA Plasmids into Urothelium of Mouse Bladder: An Electroporation-based Procedure to Efficiently Deliver DNA Plasmids into Urothelial Cells of Murine Bladder to Generate Genetically Engineered Models
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2023
This video demonstrates the delivery of DNA plasmid into the urothelium of the mouse bladder using a combination of urethral catheterization and electroporation techniques. The described procedure allows the generation of mouse bladder cancer models.
08:01
Designing CAD/CAM Surgical Guides for Maxillary Reconstruction Using an In-house Approach
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Cited by 7 •
2018
Methods for designing a computer-aided design/computer-aided manufacturing (CAD/CAM) surgical guide are shown. Cutting planes are separated, united, and thickened to easily visualize the necessary bone transfer. These designs can be three-dimensional printed and checked for accuracy.
09:17
In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents
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Cited by 1 •
2022
This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.
05:35
Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
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2022
This protocol describes a methodology to assess the function of mechanically activated ion channels in native urothelial cells using the fluorescent Ca2+ sensor GCaMP5G.
FAQs
A successful construct must coordinate cellular behavior with scaffold function. Urothelial cells or progenitor cells need a surface that supports attachment and proliferation while also enabling differentiation and formation of a continuous barrier. The scaffold therefore contributes more than physical support: its architecture and mechanical compatibility help the engineered tissue remain suited to urinary tract conditions.
Barrier formation indicates that the construct is developing the protective epithelial function expected of the urinary tract lining. This outcome depends on appropriate cell growth and differentiation rather than simple cell survival. Evaluating barrier development helps researchers determine whether a graft or laboratory model is progressing toward functional tissue suitable for repair studies or biological investigation.
Decellularized matrices and synthetic polymers provide distinct material approaches for organizing urothelial cells. Their selection can be guided by the desired tissue architecture and by mechanical compatibility with the urinary tract. Comparing these scaffold categories allows bioengineers to investigate how material context influences attachment, proliferation, differentiation, and barrier formation without treating every construct design as interchangeable.
Development generally begins by selecting urothelial cells or progenitor cells and choosing a compatible scaffold. The cells are then introduced to the scaffold as a cell-seeded graft or related construct, followed by evaluation of attachment, proliferation, differentiation, and barrier formation. These outcomes indicate whether the design is supporting tissue development under conditions relevant to the urinary tract.
Patient-specific graft design requires attention to the intended tissue architecture and the mechanical compatibility of the construct with the urinary tract. Engineers may also choose among decellularized matrices, synthetic polymers, or cell-seeded formats according to the reconstruction goal. These decisions influence how effectively cells organize and whether the resulting tissue can support the desired repair strategy.
Urothelial reconstruction can support urinary tract repair and regenerative medicine while also providing controlled platforms for studying urothelial biology. Engineered constructs may help investigate disease mechanisms and treatment responses, and their design can be adapted toward patient-specific grafts. This combination connects material engineering, cell behavior, and clinically relevant tissue modeling within bioengineering research.