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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells

Article DOI: 10.3791/64532-v • 05:35 min • September 28th, 2022
September 28th, 2022 •
1,2, 1, 1,2
1Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, 2Department of Cell Biology, University of Pittsburgh

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Summary
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This protocol describes a methodology to assess the function of mechanically activated ion channels in native urothelial cells using the fluorescent Ca2+ sensor GCaMP5G.

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Ex Vivo Analysis Mechanically Activated Ca2+ Transients Urothelial Cells Protocol Technique Study Calcium Event Native Tissue Preparations Micropipettes Capillary Glass Puller Capillary-retaining Knobs Microforge Heater-adjustment Knob Bladder Pelvic Bone Urethra Catheter Silicone Rubber Holder Pad Recording Solution Bladder Mucosa Muscular Layer Fine Forceps Urothelium Silicone Elastomer Insert Tissue Culture Dish Microscope Stage Culture Dish Incubator
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