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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells

Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells

DOI:
10.3791/64532-v

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05:35 min

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September 28, 2022

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, ,
1Renal-Electrolyte Division, Department of Medicine,University of Pittsburgh, 2Department of Cell Biology,University of Pittsburgh

Chapters

  • 00:04Introduction
  • 00:31Fabrication of Micropipettes
  • 00:56In Situ Transduction and Isolation of Bladder Mucosa
  • 01:41Mechanical Stimulation of Individual Urothelial Cells and Ca2+ Imaging
  • 03:16Data Analysis
  • 04:03Results: Analysis of Mechano-Activated Ca2+ Transients in Urothelial Cells
  • 04:49Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a methodology to assess the function of mechanically activated ion channels in native urothelial cells using the fluorescent Ca2+ sensor GCaMP5G.

Tags

Ex VivoMechanically-activatedCa2+ TransientsUrothelial CellsMicropipettesBladderUrethraCalcium Ion TransientsElectrophysiology

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