Cell Surface Adhesion
Cell Surface Adhesion - Related Videos
07:55
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
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Cited by 3 •
2017
Cell surface adhesions are central in mechanotransduction, as they transmit mechanical tension and initiate the signaling pathways involved in tissue homeostasis and development. Here, we present a protocol for dissecting the biochemical pathways that are activated in response to tension, using ligand-coated magnetic microbeads and force application to adhesion receptors.
10:52
Preparation and High-temperature Anti-adhesion Behavior of a Slippery Surface on Stainless Steel
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Cited by 3 •
2018
Slippery surfaces provide a new way to solve the adhesion problem. This protocol describes how to fabricate slippery surfaces at high temperatures. The results demonstrate that the slippery surfaces showed anti-wetting for liquids and a remarkable anti-adhesion effect on soft tissues at high temperatures.
10:24
Quantitation of Endothelial Cell Adhesiveness In Vitro
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Cited by 9 •
2015
We report an in vitro method that allows the quantitation of the actual number of adhesive cells within an endothelial cell monolayer.
09:11
Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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Cited by 5 •
2015
Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling events.
01:14
Cell Adhesion in Plants
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2023
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses. Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose, and...
FAQs
Cadherins primarily support cell-to-cell attachment, whereas integrins connect cells with the extracellular matrix or other biological surfaces. Both are membrane proteins, but their binding depends on specific ligands and their intracellular connections. This distinction lets researchers examine whether a change in tissue organization reflects altered neighboring-cell contacts, matrix attachment, or both.
Calcium, mechanical force, and signaling pathways can change how adhesion is established or maintained. Calcium regulates adhesion-related molecular interactions, while force can influence the behavior of membrane protein connections and their cytoskeletal linkages. Signaling pathways add cellular control over these responses, making adhesion dynamic rather than a fixed attachment.
Intracellular adaptor proteins provide the link between adhesion receptors at the membrane and the cytoskeleton inside the cell. That connection helps translate surface binding into an organized cellular response and allows adhesion to be considered alongside cell shape, tissue architecture, and mechanically influenced behavior. Examining this linkage can reveal how attachments affect organization.
A technique-focused workflow can use an adhesion assay to measure interactions, a coated culture substrate to present a defined biological surface, and microscopy to observe resulting cell behavior or organization. Together, these tools let investigators compare attachment under controlled experimental conditions and connect a measured adhesion response with visible changes in cells or tissues.
Cell surface adhesion experiments can examine how cells organize into tissues, migrate across biological surfaces, or participate in wound repair. They also support studies of cancer invasion, where altered attachment may be relevant to tissue behavior. By measuring or manipulating adhesion, investigators can connect surface interactions with broader changes in cellular organization and movement.
Adhesion studies help evaluate how cells interact with biomaterials and engineered tissue environments. Coated culture substrates can provide experimental surfaces for examining these interactions, while adhesion assays and microscopy supply complementary measurements and observations. The resulting information supports efforts to understand or manipulate cell organization when biological structures are designed, maintained, or studied outside their native context.