Core Extraction Technique
Core Extraction Technique - Related Videos
09:21
Sediment Core Sectioning and Extraction of Pore Waters under Anoxic Conditions
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Cited by 5 •
2016
A protocol for sectioning sediment cores and extracting pore waters under anoxic conditions in order to permit analysis of redox sensitive species in both solids and fluids is presented.
08:30
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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Cited by 53 •
2016
This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic tissue blocks.
02:10
The Nucleosome Core Particle
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2020
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
07:40
Advanced Workflow for Taking High-Quality Increment Cores - New Techniques and Devices
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Cited by 5 •
2023
Here, we present a protocol on how to avoid micro cracks in increment cores by applying a cordless drill with a torque multiplier to minimize problems when coring trees, as well as its effect on preparing long micro sections. This protocol also includes a procedure to sharpen corers in the field.
02:23
Visualizing the Effect of pH on Solubilization of the Influenza A Viral Core
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2026
Source: Stauffer, S. et al., In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation. J. Vis. Exp. (2016)This video demonstrates the effect of pH on the solubilization of influenza A viral cores. Gel electrophoresis and protein staining reveal the progressive disassembly of the core structure under acidic conditions.
FAQs
Consistency comes from controlling both the core’s diameter and its sampling position. A fixed diameter limits variation in the amount of material collected, while a known location preserves spatial comparability between specimens or experimental regions. This combination allows differences in measurements to be interpreted as biological variation more confidently rather than as consequences of inconsistent sampling.
Controlled pressure helps the coring instrument cut around the selected diameter and withdraw the sample in a consistent manner. Excessive or inconsistent force could affect how the core is removed and potentially compromise preservation of its internal organization. Maintaining controlled pressure therefore supports comparable samples across locations, specimens, or experimental conditions.
Internal structure retains the spatial organization needed to relate tissue regions to cellular or biochemical findings. When that organization remains available for examination, researchers can assess histological features and connect them with results from molecular assays. This makes the extracted material useful not only for measuring composition, but also for studying how biological structure varies within a sample.
A typical workflow begins by selecting a defined sampling location and determining the desired core diameter. The specialized coring instrument is then positioned over that region, pressure is applied in a controlled manner to cut around the selected diameter, and the resulting core is withdrawn for analysis. Consistent positioning and handling are central to producing comparable samples.
Extracted cores can support histological examination and molecular assays. Histology can evaluate tissue organization, whereas molecular analysis can examine biochemical findings from the same defined sampling strategy. Using standardized cores also enables comparisons between regions or experimental conditions, helping researchers determine whether observed differences reflect location, treatment, or other biological variation.
The method improves comparisons by standardizing two major sampling variables: the amount of material collected and its location. Cores taken with a consistent diameter from known regions provide a more controlled basis for evaluating measurements. In biological techniques, this reproducibility helps connect differences in tissue organization with corresponding cellular or biochemical findings across experimental conditions.