Hydrogel Coated Microplate
Hydrogel Coated Microplate - Related Videos
07:51
Introduction to the Microplate Reader
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2023
The microplate reader is a multimodal instrument that allows for a variety of experiments to be performed and measured simultaneously. Microplate readers can make absorbance, fluorescence and luminescence measurements. Multiwell plates are integral to the microplate reader and allow for many experiments to be performed at once. Regardless of the assay type, experiments on the plate reader utilize a standard curve to determine the experimental values. This curve uses samples of known...
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
08:33
Hydrogel Synthesis
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2023
Source: Amber N. Barron, Ashlea Patterson, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Hydrogels are a versatile class of cross-linked polymers produced through relatively simple procedures and with generally inexpensive materials. They can be formed from solution and involve a polymer backbone formed from monomer reagents, an initiator which makes the polymer reactive and a crosslinking species which binds the polymer chains...
03:01
Precise and High-Throughput Analysis of Bacterial Growth Using a Microplate Reader
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2025
Source: Kurokawa, M., et al. Precise, High-throughput Analysis of Bacterial Growth. J. Vis. Exp. (2017)This video demonstrates the high-throughput quantification of bacterial growth using a microplate reader. It outlines the steps involved in culture preparation, optical density measurement, and analysis of growth dynamics.
09:47
FRET Imaging in Three-dimensional Hydrogels
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Cited by 3 •
2016
Förster resonance energy transfer (FRET) imaging is a powerful tool for real-time cell biology studies. Here a method for FRET imaging cells in physiologic three-dimensional (3D) hydrogel microenvironments using conventional epifluorescence microscopy is presented. An analysis for ratiometric FRET probes that yields linear ratios over the activation range is described.
FAQs
The hydrated polymer network changes the physical environment at the well surface. Its water-rich, porous structure can reduce nonspecific adsorption, limit direct cell attachment, and influence how molecules move through the well. These combined effects create more controlled contact conditions for cells, proteins, and other biological samples than a surface without the same hydrogel properties.
Reducing nonspecific adsorption helps limit unintended binding of proteins or other biological samples to the well surface. This can make the intended experimental interactions easier to study and may reduce variability caused by surface-dependent sample loss. In biological techniques, that control is especially relevant when assay results depend on defined surface conditions rather than uncontrolled attachment.
The hydrogel's porous, hydrated network can influence molecular transport within each well. As samples move through or interact with this network, the local conditions experienced by cells, proteins, or other materials may differ from those at a conventional surface. Accounting for this transport behavior helps researchers interpret assay outcomes in relation to the engineered well environment.
By limiting cell attachment and providing a defined surface environment, these plates can support culture conditions in which cells organize into three-dimensional structures. This makes them useful for spheroid and organoid formation, where the experimental objective extends beyond growth on a firmly attached surface. The resulting platform supports controlled studies of cell behavior in biological techniques.
Researchers may choose hydrogel-coated wells when compound screening requires controlled interactions between cells or biological samples and the plate surface. The coating can help limit nonspecific attachment and provide more consistent conditions across wells. This is relevant when evaluating compound effects in cell-based systems, including models that use spheroids or organoids rather than only surface-attached cells.
These plates provide a defined surface context for evaluating how cells respond to engineered materials and culture conditions. Consequently, they can support research on cell behavior, biomaterials, and tissue-engineering strategies. Their controlled interface is useful for comparing biological responses and for examining whether a material or culture environment produces consistent outcomes across experimental wells.