Robotic Microinjection
Robotic Microinjection - Related Videos
05:08
Image-Guided Robotic Cell Microinjection: An Automated High-Throughput Technique for the Precise Delivery of Fluorescently-Labeled Polysaccharides Into Single Neuronal Cells in Organotypic Brain Slice Cultures
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2025
In this video, we demonstrate the robotic microinjection of fluorescently-labeled polysaccharides into pyramidal neurons in organotypic slices of mouse embryonic brain using an autoinjector. The autoinjector is a robotic platform that utilizes images acquired from a microscope for the precise delivery of biomolecules of interest into single cells.
08:12
Zebrafish Microinjection Techniques
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2023
One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...
11:40
Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
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Cited by 6 •
2021
This protocol demonstrates the use of a robotic platform for microinjection into single neural stem cells and neurons in brain slices. This technique is versatile and offers a method of tracking cells in tissue with high spatial resolution.
12:10
Microinjection of Xenopus Laevis Oocytes
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Cited by 14 •
2009
Here we demonstrate cytoplasmic microinjection of Xenopus laevis oocytes with a nuclear import substrate, as well as preparation of the injected oocytes for visualization by thin-sectioning electron microscopy.
11:01
SSVEP-based Experimental Procedure for Brain-Robot Interaction with Humanoid Robots
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Cited by 12 •
2015
The overall goal of this method is to establish an SSVEP-based experimental procedure by integrating multiple software programs to enable the study of brain-robot interaction with humanoid robots, which is prospective in assisting the sick and elderly as well as performing unsanitary or dangerous jobs.
FAQs
The robotic micromanipulator guides the fine glass needle toward a selected cell, embryo, or other microscopic target, while controlled pressure, displacement, or fluid flow regulates delivery. Separating needle positioning from fluid control helps coordinate membrane entry and substance release. This arrangement supports consistent handling when researchers must introduce very small amounts into precisely selected locations.
Automation can standardize needle movement and the forces or flows used during delivery, reducing variation between injection attempts. It also limits operator fatigue, which may otherwise affect consistency during repeated procedures. These advantages are particularly relevant when experiments require many comparable injections or when reliable delivery is important for interpreting gene-function or developmental outcomes.
The material determines which biological question the procedure can address. DNA or RNA can support studies of gene activity and gene function, whereas proteins or chemical agents provide other ways to alter or examine cellular processes. Selecting among these cargo types allows the same delivery platform to support transgenesis, cell engineering, or disease-modeling research.
A typical workflow begins by positioning the microscopic target and aligning a fine glass needle with it using the robotic micromanipulator. The system then uses controlled pressure, displacement, or fluid flow to move the selected material through the cell membrane. After delivery, the setup can be used for repeated injections under comparable positioning and control conditions.
Researchers may choose this approach when experiments require material delivery into individual cells, embryos, or other microscopic targets. Its applications include transgenesis, gene-function studies, assisted reproduction, and production of genetically modified organisms. The technique also supports developmental biology, cell engineering, and disease modeling when precise, repeatable delivery is needed.
Robotic microinjection can improve targeting accuracy, consistency, and throughput compared with less automated handling. These characteristics may make results more reproducible across injections and reduce fatigue during extended experiments. In biological techniques, the resulting control is valuable for studies that compare cellular responses, examine development, or generate genetically modified systems.