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Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

DOI:
10.3791/61599-v

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11:40 min

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January 21, 2021

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, , , ,
1Department of Biomedical Engineering,University of Minnesota, 2Department of Biomedical Engineering,Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering,University of Minnesota, 6Graduate Program in Neuroscience,University of Minnesota

Chapters

  • 00:05Introduction
  • 01:02Tissue Slice Preparation
  • 04:04Microinjection
  • 07:05Tissue Culture and Tissue Slice Processing for Immunofluorescence
  • 09:39Results: Robotic Microinjection into Apical Progenitors and Neurons
  • 10:57Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates the use of a robotic platform for microinjection into single neural stem cells and neurons in brain slices. This technique is versatile and offers a method of tracking cells in tissue with high spatial resolution.

Tags

Keywords: Single Neural Stem CellsNeuronsBrain SlicesRobotic MicroinjectionVisual DemonstrationAuto-injector SoftwareMicroinjection PipettesAgaroseSCMSIMTyrode’s SolutionFluorescent TracerTelencephalonEmbryonic BrainMeninges

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