Synthetic Resin Mounting
Synthetic Resin Mounting - Related Videos
07:07
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
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Cited by 2 •
2015
The synthesis of asymmetric species of ferrocene is challenging using solution techniques. This report focuses on the methods carried out to produce a ferrocene-biotin bioconjugate using facile and clean reactions accomplished via solid-phase synthesis. Incorporation of a thiolate moiety is shown to impart the ability for immobilization on gold surfaces.
16:30
Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments
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Cited by 2 •
2007
In this report, we propose a new application of light-curing dental resins for mounting base of electrodes or microdialysis probes in chronic experiments. This material allows direct bonding to the cranium.
02:38
Anion Exchange Resin–Based Detection of Viruses from Bioaerosols
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2026
Source: Schaeffer, J. W., Chandler, et al. Detection of Viruses from Bioaerosols Using Anion Exchange Resin. J. Vis. Exp. (2018).This video demonstrates the detection of viruses from bioaerosols using an anion exchange resin. A liquid impinger containing the resin is used to capture viruses from the air. The resin is collected and treated with a virus lysis buffer containing carrier RNA. The lysate is transferred to a microcentrifuge tube for viral RNA isolation, followed by qRT-PCR to amplify...
07:51
Acrylic Resin Molding Based Head Fixation Technique in Rodents
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Cited by 4 •
2016
Traditional head fixation techniques have used metallic frames attached on the skull as an interface for head fixation apparatus. This protocol demonstrates a frameless, acrylic resin molding based head fixation technique for behavioral tasks in rodents.
16:11
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
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Cited by 380 •
2013
This five-day protocol outlines all steps, equipment, and supplemental software necessary for creating and running an efficient endogenous Escherichia coli based TX-TL cell-free expression system from scratch. With reagents, the protocol takes 8 hours or less to setup a reaction, collect, and process data.
FAQs
The mounting medium begins as a synthetic polymer dissolved in an organic solvent. After placement beneath the coverslip, the solvent evaporates or the resin cures, converting the fluid medium into a hardened polymer layer. This change secures the coverslip and stabilizes the stained section, allowing the slide to withstand handling during repeated microscopic examination and long-term storage.
Dehydration and clearing prepare the tissue section to receive the resin medium. The overview identifies these steps as preceding placement of the specimen in polymer dissolved in an organic solvent, so they are essential parts of the workflow rather than optional handling steps. Their position before mounting supports proper preparation for hardening, optical clarity, and preservation under the coverslip.
Once hardened, the resin provides optical clarity for transmitted light microscopy while holding the coverslip securely over the specimen. This stable optical arrangement helps preserve the visibility of cellular architecture, disease-related changes, and histological stain distribution. Because the section remains protected beneath the coverslip, observations and images can be compared over time without relying on a freshly prepared mount.
Its durability results from two linked features: the polymer medium hardens after solvent evaporation or curing, and the hardened layer protects the section beneath a secured coverslip. Unlike a preparation intended only for immediate viewing, this arrangement supports long-term storage and archival documentation. It is therefore useful when stained tissue must remain available for later examination or image comparison.
The process begins with a stained biological section, followed by dehydration and clearing. The section is then placed in a synthetic polymer medium dissolved in an organic solvent, and a coverslip is secured over it. Solvent evaporation or curing hardens the resin. The resulting slide is suited to transmitted light microscopy and durable preservation.
In biological techniques, this approach supports microscopic study of tissue organization and pathology. Mounted sections can be examined for cellular architecture, disease-related changes, and the distribution of histological stains. The permanent preparation also benefits projects that require durable slide collections, repeated image comparison, or archival documentation rather than a single short-term observation.