70 Micron Cell Strainer
70 Micron Cell Strainer - Related Videos
10:36
Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System
0 Views •
Cited by 6 •
2015
Here, we present a protocol to fabricate freely-suspended, micron/sub-micron scale polymer fibers and “web-like” structures generated via automated direct writing procedure by means of a 3-axis dispensing system.
05:02
Mechanical Micronization of Lipoaspirates for Regenerative Therapy
0 Views •
Cited by 18 •
2019
Here, we present a protocol to obtain stromal vascular fraction from adipose tissue through a series of mechanical processes, which include emulsification and multiple centrifugations.
08:51
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
0 Views •
Cited by 9 •
2008
We used synchrotron X-ray tomography at the European Synchrotron Radiation Facility (ESRF) to non-invasively produce 3D tomographic datasets with a pixel-resolution of 0.7µm. Using volume rendering software, this allows the reconstruction of internal structures in their natural state without the artefacts produced by histological sectioning.
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
0 Views •
Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
09:54
Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
0 Views •
Cited by 3 •
2021
This work presents a bottom-up approach to the engineering of local magnetic forces for control of neuronal organization. Neuron-like cells loaded with magnetic nanoparticles (MNPs) are plated atop and controlled by a micro-patterned platform with perpendicular magnetization. Also described are magnetic characterization, MNP cellular uptake, cell viability, and statistical analysis.
FAQs
Separation depends on particle size relative to the membrane openings. Individual cells and sufficiently small particles pass through, whereas larger cell aggregates, undissociated tissue, and debris remain on the membrane. This size-based selection reduces the amount of oversized material entering the collected suspension and helps create a more consistent sample for downstream biological analysis.
Cell clumps can make a suspension less uniform and introduce variability during pipetting, counting, microscopy, or instrument-based analysis. Passing the sample through the strainer reduces larger aggregates before those steps occur. The resulting suspension is easier to handle and can support more consistent measurement of primary cells and other biological samples.
The main determinant is the size of each cell, particle, or tissue fragment compared with the 70-micron openings. Small individual cells can pass, while larger aggregates and incompletely dissociated tissue are retained. Consequently, the quality of the preceding tissue dissociation and the amount of debris in the sample influence the material recovered after filtration.
Pipetting moves the sample but does not provide the same size-based removal of larger aggregates, undissociated tissue, and debris. Filtration adds a physical selection step before analysis or culture. That step can make the suspension more uniform, improve handling during pipetting, and reduce the chance that oversized material interferes with later sample processing.
Researchers can use it after tissue dissociation and before downstream procedures that benefit from a uniform cell suspension. The overview identifies flow cytometry, cell counting, microscopy, and cell culture as relevant uses. Positioning filtration within sample preparation helps remove retained material before the sample reaches these analytical or cultivation steps.
Filtration can produce a suspension with fewer large aggregates and less debris, which supports more consistent pipetting and sample handling. It may also help protect instruments from blockages when prepared material is introduced for analysis. These benefits are particularly relevant when working with primary cells, whose samples may contain incompletely dissociated tissue.
For microscopy, reducing clumps and debris can make the prepared suspension more uniform for observation. In cell culture workflows, removing larger unwanted material provides a cleaner starting suspension for handling and further processing. The strainer therefore functions as a sample-preparation step that improves consistency without replacing the biological procedures performed afterward.