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JoVE Journal
Bioengineering
Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

Full Text
5,275 Views
09:54 min
July 14, 2021

DOI: 10.3791/62013-v

Ganit Indech1,4, Reut Plen2,4, Dafna Levenberg2,4, Naor Vardi1,4, Michal Marcus2,4, Alejandra Smith2,4, Shlomo Margel3,4, Orit Shefi2,4, Amos Sharoni1,4

1Department of Physics,Bar-Ilan University, 2Faculty of Engineering,Bar-Ilan University, 3Department of chemistry,Bar-Ilan University, 4The Institutes of Nanotechnology & Advanced Materials,Bar-Ilan University

This work presents a bottom-up approach to the engineering of local magnetic forces for control of neuronal organization. Neuron-like cells loaded with magnetic nanoparticles (MNPs) are plated atop and controlled by a micro-patterned platform with perpendicular magnetization. Also described are magnetic characterization, MNP cellular uptake, cell viability, and statistical analysis.

Our approach makes it possible to control neural network formation using magnetic manipulations. This is an effective tool for in vitro studies of networks and offers novel therapeutic direction for biointerfacing devices. With this technique we can control both cell's location and growth at the micron scale, allowing flexible design of the magnetic pattern and thus the network organization.

An efficient and non-toxic magnetic nanoparticle steriloptic is crucial. To succeed, appropriate magnetic nanoparticles are needed. The important features are the size, coating, and situation magnetization value.

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