Adipocytes
Adipocytes - Related Videos
08:34
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
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Cited by 5 •
2016
Adipose tissue (AT) can influence whole body homeostasis, therefore understanding the molecular mechanisms of adipocyte differentiation and function is of importance. We provide a protocol for gaining new insights into these processes by analyzing adipocyte homeostasis, differentiation and hypoxia exposure as a model for induced adipocyte apoptosis.
03:03
In Vitro Mouse Bone Marrow Stromal Cell Differentiation into Adipocytes
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2025
This video demonstrates the differentiation of bone marrow stromal cells (BMSCs) into adipocytes. In the two-step process, in the presence of specific growth factors and hormones, undifferentiated BMSCs first differentiate into preadipocytes. The second step involving treatment with glucose and insulin leads to the formation of mature adipocytes with multiple lipid droplets.
07:35
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
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Cited by 16 •
2015
Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
10:40
Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
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2025
The protocol uses an adipocyte-specific CD63-GFP reporter mouse line to visualize and quantify the secretion of adipocyte-derived extracellular vesicles (EVs) and demonstrate their uptake by progenitor cells, revealing a paracrine pathway within adipose tissue.
08:28
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Cited by 5 •
2017
Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).
FAQs
Adipogenesis proceeds as precursor cells develop into adipocytes. During this process, the cells accumulate lipid droplets, creating the intracellular structure needed for triglyceride storage. Tracking this transition allows biologists to examine how cell development becomes linked to later regulation of energy balance and metabolism at the whole-body level.
Catecholamines can signal increased energy demand and stimulate lipolysis, the process through which adipocytes release stored fatty acids. This response connects hormonal communication with fuel availability, allowing researchers to examine how adipose tissue contributes to changing metabolic needs rather than functioning only as a passive energy reservoir.
White, brown, and beige adipocytes provide distinct cellular contexts for studying energy balance and thermogenesis. Comparing these populations helps researchers investigate how adipose tissue may store energy, participate in heat-producing processes, or influence metabolism differently. Their comparison is particularly relevant to research on obesity and other metabolic disorders.
Adipocytes act as endocrine cells by secreting adipokines, signaling molecules that influence appetite, inflammation, insulin sensitivity, and communication between tissues. Measuring or examining these signals helps connect adipocyte activity with whole-body physiology and provides a framework for studying how altered adipose function may affect metabolic health.
A useful investigation can follow several linked features: precursor-cell development during adipogenesis, lipid-droplet accumulation, catecholamine-associated lipolysis, and adipokine secretion. Comparing white, brown, and beige adipocytes adds cellular context. Together, these observations relate cell behavior to energy balance, thermogenesis, tissue communication, and metabolic regulation.
Adipocytes connect energy storage, fatty-acid release, endocrine signaling, and whole-body metabolism. Studying their development, hormonal responses, adipokines, and cellular differences can clarify processes associated with obesity and type 2 diabetes. This knowledge also supports research into potential strategies for treating metabolic disease by targeting adipose-related mechanisms.