Journal
/
Developmental Biology
/
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

DOI:
10.3791/55818-v

•

08:28 min

•

June 30, 2017

•

, , ,
1Department of Large Animal Clinical Sciences,Michigan State University, 2Department of Pharmacology and Toxicology,Michigan State University

Chapters

  • 00:05Title
  • 00:50Adipocyte Progenitors Isolation
  • 02:52Magnetic Activated Cell Sorting
  • 05:13Cell Culture and Adipogenesis Induction
  • 06:35Results: Representative Adipocyte Progenitor Cells (APCs) Differentiation Analysis
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

Tags

Keywords: Adipocyte Progenitor CellsMagnetic Activated Cell SortingAdipogenesisStromal Vascular FractionCollagenase DigestionCell FiltrationErythrocyte Lysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55818?article_type=v

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
check_url/55818?article_type=v

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
check_url/55818?article_type=v

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
check_url/55818?article_type=v

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
check_url/55818?article_type=v

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
check_url/55818?article_type=v

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
check_url/55818?article_type=v

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
check_url/55818?article_type=v

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
check_url/55818?article_type=v

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
check_url/55818?article_type=v

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Read Article