Advanced Cell Culture
Advanced Cell Culture - Related Videos
07:14
Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
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Cited by 8 •
2021
An enhanced platform for whole-embryo culture allows continuous and robust ex utero development of postimplantation mouse embryos for up to six days, from pregastrulation stages until advanced organogenesis. In this protocol, we detail the standard procedure for successful embryo culture using static plates and rotating bottle systems.
05:47
Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development
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Cited by 18 •
2015
Viewing and accessing the chicken embryo during development can be challenging. We have developed an ex ovo method that is simple, cost effective, and can easily be used in a classroom or research setting. This method provides access to the embryo into late stages of embryonic development (HH 40).
08:05
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
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Cited by 3 •
2016
Here is a protocol to grow pluripotent stem cells (PSC) and neural stem cells (NSC) in an enclosed cell culture system that permits maximum sterility and reproducibility, replacing the traditional biosafety cabinet and incubator. This equipment meets clinical good manufacturing practice (cGMP) and clinical good lab practice (cGLP) guidelines.
06:23
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
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Cited by 14 •
2019
Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal tract.
FAQs
Regulating nutrient supply, temperature, gas composition, extracellular matrix, and cell-cell interactions can shape how cells behave in culture. These factors help researchers create environments that better reflect living tissues rather than relying on a single simplified condition. The resulting systems support closer examination of cellular behavior, differentiation, disease processes, and responses to experimental treatments.
Three-dimensional systems organize cells in arrangements that more closely resemble tissue structure, while conventional two-dimensional cultures grow cells on a flat surface. This difference can affect how cells interact with one another and with the extracellular matrix. Consequently, three-dimensional models may offer more biologically relevant information when studying differentiation, disease processes, or treatment responses.
The extracellular matrix and cell-cell interactions provide environmental signals that influence cellular behavior within a culture system. Advanced approaches regulate these features deliberately so researchers can examine cells in conditions that better approximate tissue organization. This is especially relevant when investigating differentiation, tissue engineering, regenerative research, or pathological processes that depend on interactions among cells and their surroundings.
These formats add different levels of biological or environmental complexity. Co-cultures bring multiple cell populations together, organoids create three-dimensional tissue-like models, and dynamic bioreactors introduce controlled culture conditions through a more active system. Selecting among them allows researchers to match the model to questions about cellular interactions, tissue behavior, disease, drug responses, or biomaterials.
Researchers should determine which culture format and environmental variables best match the biological question. Important considerations include nutrient supply, temperature, gas composition, extracellular matrix, cell-cell interactions, and whether a three-dimensional, co-culture, organoid, or dynamic bioreactor system is appropriate. This planning helps align the model with the tissue process or response under investigation.
It is useful when conventional culture does not adequately represent the biological process being studied. Researchers apply these systems to investigate cellular behavior, differentiation, disease mechanisms, responses to drugs or biomaterials, tissue engineering, and regenerative research. Their greater biological relevance can support experimental models of complex physiological and pathological processes.
Depending on the model and conditions selected, researchers can examine how cells behave, differentiate, interact, and respond to external interventions. These systems also support investigation of disease processes and evaluation of responses to drugs or biomaterials. In biology, such information contributes to tissue engineering, regenerative research, and the study of complex tissue-related mechanisms.