Adherent Mammalian Cells
Adherent Mammalian Cells - Related Videos
09:20
In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
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Cited by 29 •
2010
In situ subcellular fractionation of mammalian cells on microscope coverslips allows the visualisation of protein localisation.
02:59
Bead Loading to Introduce Nucleic Acids Into Adherent Cultured Cells: A Technique to Load Fluorescent Protein-Encoding Plasmids Into Adherent Mammalian Cells
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2025
In this video, we describe the procedure to introduce nucleic acids into adherent mammalian cells using bead loading.
05:30
Clonogenic Assay: Adherent Cells
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Cited by 228 •
2011
The applicability of the clonogenic assay for evaluating reproductive viability has been established for more than 50 years. Here we demonstrate the general procedure for performing the clonogenic assay with adherent cells.
02:52
Expansion of Mutant Vaccinia Virus in Mammalian Cells
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2026
Source: Yuan, M., et al. A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors. J. Vis. Exp. (2016)This video demonstrates the expansion of plaque-derived recombinant virus in adherent mammalian cells. The virus carries a fluorescent reporter in place of a virulent gene, enabling infection tracking. Infected cells are harvested for PCR verification of the genetic modification.
05:57
In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
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Cited by 81 •
2011
This protocol is a simple bacterial adhesion assay consisting in counting the numbers of bacterial colony forming units that are adhered onto cultured cells. The assay is robust, independent of the adhesin studied, and numerous variations are used in most laboratories working on bacterial pathogenesis.
FAQs
Attachment depends on the properties of the solid surface, including whether plastic has been treated or the vessel has been coated with extracellular matrix. These features influence how cells attach, spread, and maintain viable cultures. Consistent substrate preparation is therefore important when comparing morphology, proliferation, signaling, or other experimental responses across conditions.
Following attachment, cells extend cytoskeletal structures that support spreading and organization on the substrate. They can also establish contacts with neighboring cells, linking surface interactions to tissue-like behavior in culture. These changes provide measurable context for studies of morphology, cell signaling, gene expression, and tissue-specific function.
Confluence, the proportion of the vessel surface occupied by cells, can affect how consistently cultures grow and respond experimentally. Temperature, nutrient availability, pH, and gas exchange must also remain controlled because they support survival, proliferation, and stable cell behavior. Monitoring these variables improves reproducibility in cell biology experiments and drug testing.
Passaging begins by releasing cells from the culture surface when a fresh culture is needed. Researchers may use enzymatic or mechanical detachment, then transfer the cells into another suitable culture vessel under controlled conditions. Managing this step alongside sterility and confluence helps maintain viable populations and supports repeatable expansion for subsequent experiments.
These cultures are useful when researchers need to examine cell signaling, morphology, gene expression, toxicity, infection, or tissue-specific function under controlled conditions. Their dependence on a defined surface makes changes in attachment, spreading, and cell-cell contact experimentally relevant. Applications include drug testing, disease modeling, and regenerative biology.
Experiments can reveal how cells change their morphology, signaling, gene expression, survival, or proliferation in response to controlled culture conditions. Depending on the model, researchers can also investigate toxicity, infection, or tissue-specific behavior. Careful control of substrate, confluence, sterility, and the culture environment strengthens interpretation and improves comparison between experiments.