Agilent Bioanalyzer
Agilent Bioanalyzer - Related Videos
09:15
DNA-based Fish Species Identification Protocol
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Cited by 9 •
2010
This publication describes how to use the Agilent Fish Species Identification System to identify the species of a fish by extracting DNA and performing PCR and RFLP analysis.
14:13
A Training Program Using an Agility Ladder for Community-Dwelling Older Adults
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Cited by 11 •
2020
The aim of this study is to present an agility training program for older people. The feasibility of this program is described, and the training protocol demonstrated by video imaging.
08:37
Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer
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2024
Alcohol misuse impairs alveolar macrophage (AM) immunity due to suppressed mitochondrial respiration and bioenergetics. We recently demonstrated that ethanol (EtOH) exposure increases glutamine dependency for mitochondrial respiration in AMs. Herein, methods are provided to determine the usage of glutamine for mitochondrial respiration in EtOH-treated AMs using an extracellular flux bioanalyzer.
08:55
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
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Cited by 13 •
2012
This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.
08:52
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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Cited by 8 •
2011
A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.
FAQs
The system directs a sample through channels in a disposable lab-on-a-chip device, where electrophoretic separation resolves components according to their movement through the microfluidic environment. Detection of the separated components produces an electropherogram rather than a single bulk measurement. This format allows researchers to examine distributions of nucleic-acid or protein components using small sample volumes.
An electropherogram shows how detected material is distributed across component sizes, allowing researchers to evaluate concentration together with fragment distribution and integrity. For RNA, an abnormal pattern can indicate degradation; for DNA or cDNA, the distribution can reveal whether fragments have the expected size characteristics. These combined measurements provide more informative quality control than concentration alone.
Microfluidic channels support separation and detection while using only small sample volumes, which is valuable when biological material is limited. The disposable chip format also contributes to a standardized analysis environment for each run. Together, these features help researchers assess sample quality consistently before committing samples to sequencing or other downstream molecular workflows.
Results can expose degraded RNA or DNA and can show fragment distributions that are improperly sized for a planned workflow. Detecting these problems before downstream processing gives researchers an opportunity to evaluate sample suitability rather than relying only on the sample's measured concentration. This quality-control step can improve the reliability of subsequent molecular results.
It is especially useful before sequencing and other molecular workflows that depend on suitable nucleic-acid quality or fragment size. RNA samples can be examined for integrity, while DNA and cDNA samples can be checked for their fragment distributions. Protein samples are also within the system's analytical scope, extending its use beyond nucleic-acid quality control.
Researchers can use the generated electropherograms and quantitative measurements to determine whether a library or related nucleic-acid preparation has an appropriate fragment distribution and sufficient quality for further processing. Identifying improperly sized or degraded material before sequencing helps standardize sample selection and reduces the risk that unsuitable preparations will weaken the reliability of downstream data.