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JoVE Journal
Immunology and Infection

This content is Open Access.

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
 

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

Article doi: 10.3791/3293
September 28th, 2011 Usage Statistics

Summary September 28th, 2011

A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.

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