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RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
RNA Isolation of <em>Pseudomonas aeruginosa</em> Colonizing the Murine Gastrointestinal Tract
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract

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08:52 min

September 28, 2011

DOI:

08:52 min
September 28, 2011

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Summary

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A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.

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