Agrobacterium Tumefaciens
Agrobacterium Tumefaciens - Related Videos
05:01
Agrobacterium-Mediated Genetic Transformation: A Method to Genetically Transform the Rice Genome via Genetically Engineered Agrobacterium tumefaciens
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2025
In this video, we demonstrate genetic transformation of the rice genome using an Agrobacterium tumefaciens binary vector. This bacterium facilitates the transfer of a foreign DNA plasmid using Agrobacterium virulence proteins.
07:50
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
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Cited by 7 •
2023
Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.
08:00
Agrobacterium tumefaciens-Mediated Genetic Engineering of Green Microalgae, Chlorella vulgaris
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Cited by 3 •
2023
This protocol outlines the utilization of Agrobacterium tumefaciens-mediated transformation (AMT) for integrating gene(s) of interest into the nuclear genome of the green microalgae Chlorella vulgaris, leading to the production of stable transformants.
08:31
Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
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Cited by 7 •
2019
Here, we present two protocols to transform potato plants. The Agrobacterium tumefaciens transformation leads to a complete transgenic plant while the Agrobacterium rhizogenes produces transgenic hairy roots in a wild type shoot that can be self-propagated. We then detect promoter activity by GUS staining in the transformed roots.
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Preparation of Agrobacterium Culture for Gene Delivery into Plant Cells
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2026
Source: Shi, J., et al., Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens. J. Vis. Exp. (2020)This video demonstrates the procedure for preparing an Agrobacterium tumefaciens culture by inducing virulence gene expression with acetosyringone, followed by antibiotic removal through washing and resuspension, to generate a transformation-ready suspension for gene delivery into plant tissues.
FAQs
When Agrobacterium senses compounds released by wounded plant tissue, it activates a virulence response. Virulence proteins then process T-DNA carried on the tumor-inducing Ti plasmid and support its delivery into the plant cell. This injury-linked sensing connects environmental information to the molecular events that begin genetic reprogramming.
The Ti plasmid provides the genetic framework for transfer, while T-DNA is the segment that moves into the plant cell. Virulence proteins process that segment before delivery, separating the transferable DNA functionally from the larger plasmid. This organization enables bacterial genetic information to reach plant cells and helps explain why the plasmid is central to both disease development and transformation research.
Integration of T-DNA into the plant genome gives the transferred DNA a lasting position within the cell’s genetic material. The inserted information alters hormone production, changing how infected cells behave and contributing to crown gall disease. This link between DNA integration, hormone regulation, and abnormal growth makes the system useful for examining how genetic changes can reshape plant development.
In natural infection, the transferred DNA produces disease-associated reprogramming in plant cells. In Agrobacterium-mediated transformation, researchers use the same delivery principle to introduce a selected gene into a plant for a chosen experimental or agricultural purpose. The shared mechanism is DNA transfer, but the intended outcome shifts from disease-associated changes to genetic modification for research or crop development.
A conceptual transformation workflow begins with Agrobacterium sensing compounds associated with wounded plant tissue, followed by virulence-protein processing of T-DNA, delivery into a plant cell, and integration into the plant genome. For biotechnology, the transferable DNA is used to introduce a selected gene rather than relying only on the disease-associated outcome. This sequence links bacterial sensing and DNA delivery to a genetically altered research plant.
The system supports production of transgenic crops, disease-resistance lines, and research plants used to study gene function. Its value comes from combining DNA delivery with integration into the plant genome, allowing selected genetic information to be introduced into plants. These applications extend the bacterium’s biological role from causing disease to providing a practical route for plant genetic engineering.
Agrobacterium tumefaciens provides a model for studying plant-microbe interactions because its infection depends on signals from wounded tissue and leads to genetic changes in the host. The system also lets researchers connect bacterial virulence, DNA transfer, plant hormone production, and gene function within one experimental framework. That combination explains its importance in plant biology beyond crown gall disease.