Albumin Culture Dish

An albumin culture dish is a laboratory culture vessel prepared with albumin, a soluble protein that helps create a biologically compatible environment for cells, tissues, or embryos. Albumin can coat the dish surface or supplement the culture medium, where it binds small molecules, limits nonspecific adsorption, and helps stabilize conditions during incubation. In biology, these dishes support controlled in vitro culture and observation, including studies of cell behavior, embryo development, fertilization, and cellular responses to experimental conditions. Their use helps improve culture consistency while reducing interactions between biological samples and the underlying plastic surface.

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Research

JoVE EoE - Neuronal Culture Techniques

Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture

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2025

This video demonstrates constructing a three-compartment cell culture system using collagen and non-reactive grease. This system is used to observe interactions between glioma cells and axons in real-time.

A Functional Motor Unit in the Culture Dish: Co-culture of Spinal Cord Explants and Muscle Cells

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Cited by 16 •

2012

Cultured muscle cells are an inadequate model to recapitulate innervated muscle in vivo. A functional motor unit can be reproduced in vitro by innervation of differentiated human primary muscle cells using rat embryo spinal cord explants. This article describes how co-cultures of spinal cord explants and muscle cells are established.

Microtiter Dish Biofilm Formation Assay

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Cited by 2243 •

2011

The assay describes a rapid means to measure early biofilm formation in bacteria and fungi. This method uses a microtiter plate as the substratum for microbial biofilm formation, and the biofilm is visualized using crystal violet strain. The assay provides either a qualitative or quantitative assay for early biofilm formation.

Production of Cardiac Extracellular Matrix from Adult Human Fibroblasts for Culture Dish Coating

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Cited by 1 •

2024

Fibroblasts isolated from the adult human heart were cultured to confluence on gelatin-coated dishes to produce the myocardium-specific extracellular matrix. After decellularization, this substrate can be used for the culture and study of other cardiac cells and cell-matrix interactions.

Method for Culture of Early Chick Embryos ex vivo (New Culture)

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Cited by 9 •

2008

This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.

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