Anaerobic Ecosystems

Anaerobic ecosystems are biological communities that function without molecular oxygen, allowing organisms to survive and interact in oxygen-depleted environments such as sediments, wetlands, animal intestines, and wastewater systems. Instead of aerobic respiration, microorganisms use alternative electron acceptors or fermentation to conserve energy, producing compounds such as organic acids, sulfide, carbon dioxide, and methane; metabolic partnerships can link processes through syntrophy and methanogenesis. Studying these ecosystems reveals how microbial communities drive carbon, nitrogen, and sulfur cycling, influence greenhouse-gas production, and support applications in wastewater treatment, renewable bioenergy, agriculture, and environmental biotechnology.

Anaerobic Ecosystems - Related Videos

Education

JoVE Core - Biology

What is an Ecosystem?

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2019

Overview An ecosystem is the interaction between all abiotic and biotic factors in an environment and can be classified as terrestrial or aquatic. Terrestrial ecosystems are categorized based on the climate, including annual temperature, rainfall, and seasonality. Aquatic ecosystems are separated further into freshwater and marine, and then by depth, which influences water temperature and the amount of sunlight that penetrates the water. Terrestrial Ecosystems Terrestrial ecosystems are...

Research

JoVE Journal - Immunology and Infection

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

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Cited by 100 •

2013

Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.

Nutrients in Aquatic Ecosystems

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2023

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Nitrogen and phosphorus are essential plant nutrients found in aquatic ecosystems and both are monitored as a part of water quality testing because in excess amounts they can cause significant water quality problems. Nitrogen in water is measured as the common form nitrate (NO3-) that is dissolved in water and readily absorbed by photosynthesizers such as algae. The common form of phosphorus measured is phosphate...

Preparing an Anaerobic Sludge Culture under Oxygen-Free Conditions

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2025

Source: Wagner, A. O.,et al., Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions. J. Vis. Exp. (2019)This video demonstrates the preparation and inoculation of anaerobic cultures using oxygen-free sludge under strictly controlled, deoxygenated conditions.

Anaerobic Digestion of Bioplastics for Biogas Production

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2025

Begin with an anaerobic digester, a sealed, oxygen-free reactor seeded with a synthetic sludge that mimics the settled solids from wastewater.The sludge contains mesophilic anaerobic microbes, a mixed microbial community.These microbes thrive at moderate temperatures and drive the digestion of the sludge’s organic matter.Continuously mix the sludge to sustain the bacterial activity and promote digestion.Later, add bioplastics, which are biodegradable polymers, into the sludge.During incubation,...

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