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JoVE Journal
Immunology and Infection

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Antibiotic Dereplication Using the Antibiotic Resistance Platform
 

Antibiotic Dereplication Using the Antibiotic Resistance Platform

Article DOI: 10.3791/60536-v 10:49 min
October 17th, 2019

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Summary October 17th, 2019

We describe a platform that utilizes a library of isogenic antibiotic resistant Escherichia coli for the dereplication of antibiotics. The identity of an antibiotic produced by bacteria or fungi can be deduced by the growth of E. coli expressing its respective resistance gene. This platform is economically effective and time-efficient.

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