Aortic Intima Exposure
Aortic Intima Exposure - Related Videos
06:43
Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus
0 Views •
Cited by 51 •
2013
We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.
12:17
Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots
0 Views •
Cited by 4 •
2017
Full-root aortic valve replacement by stentless aortic xenograft is a viable option in patients with small aortic roots. We describe, a technique for the full-root implantation of stentless aortic xenografts, with emphasis on the management of the proximal suture line and coronary anastomoses, and discuss its limitations and alternative options.
09:12
Aortic Ring Assay
0 Views •
Cited by 42 •
2009
Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.
08:54
Reliable Creation of a Murine Abdominal Aortic Aneurysm Model Using Elastin Sensitization and Perivascular Elastase
0 Views •
2026
This protocol provides a repeatable and reliable method for inducing abdominal aortic aneurysms (AAAs) in C57BL/6 mice using elastin sensitization and perivascular elastase application, as an AAA model that incorporates an immune-sensitization component.
10:12
Near-infrared Fluorescence Imaging of Abdominal Aortic Aneurysms
0 Views •
2023
Source: Arvin H. Soepriatna1, Kelsey A. Bullens2, and Craig J. Goergen1 1 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 2 Department of Biochemistry, Purdue University, West Lafayette, Indiana Near-infrared fluorescence (NIRF) imaging is an exciting optical technique that utilizes fluorescent probes to visualize complex biomolecular assemblies in tissues. NIRF imaging has many advantages over conventional imaging methods for noninvasive imaging of diseases.
FAQs
Intact exposure permits examination of the endothelial lining, whereas disruption can reveal the subendothelial matrix beneath it. That distinction matters because the matrix may interact with blood components, creating a way to relate loss of endothelial integrity to processes such as thrombosis or vascular injury. Researchers can therefore compare surface condition with changes deeper in the aortic wall.
By bringing the intimal surface into view, the workflow supports separate assessment of endothelial integrity, vascular lesions, and inflammatory changes. These features represent different observations: the endothelial lining can be examined for alteration, lesions indicate structural pathology, and inflammatory changes provide evidence of a local biological response. Examining them together helps connect surface appearance with changes in the aortic wall.
Exposure focuses analysis on the innermost wall rather than leaving the aortic surface beneath surrounding tissues. This focused access is important when the research question concerns endothelial integrity or interaction between the subendothelial matrix and blood components. It also distinguishes direct surface assessment from broader examination of the aortic wall, where details of the intima may not be readily accessible.
An experimental workflow begins by carefully separating or removing tissues surrounding the aorta. The intimal surface is then visualized or physically accessed, with disruption performed when examination of the subendothelial matrix is required. The prepared area can subsequently undergo imaging, histological analysis, or immunostaining. This sequence links tissue preparation to the specific structural or cellular feature under investigation.
Imaging provides visual assessment of the exposed surface, while histological analysis examines tissue structure. Immunostaining adds assessment of selected features within the prepared specimen. Together, these approaches can document endothelial integrity, lesions, or inflammatory changes from complementary perspectives. The appropriate readout depends on whether the study emphasizes surface appearance, wall organization, or specific features revealed within the tissue.
In biology research, this approach is useful when investigators need direct evidence from the aortic wall in studies of vascular injury, atherosclerosis, thrombosis, or other mechanisms affecting cardiovascular health. It can connect visible or tissue-level changes with the condition of the intimal surface. The resulting observations support investigations focused on injury, lesions, inflammation, or interactions involving blood components.