Avian Embryo Manipulation
Avian Embryo Manipulation - Related Videos
08:40
Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model
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Cited by 90 •
2011
Using quantitative PCR, we demonstrate how the well-established chick CAM model can be used to quantitatively analyze the metastasis of human tumor cells to distant organs.
11:19
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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Cited by 11 •
2016
A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.
08:52
Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
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2019
In this method, embryonic cardiac tissues are surgically microdissected, dissociated, fluorescently labeled, and implanted into host embryonic tissues. This provides a platform for studying the individual or tissue level developmental organization under ectopic hemodynamic conditions, and/or altered paracrine/juxtacrine environments.
11:27
Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve
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Cited by 3 •
2013
Here we describe a microdissection technique followed by fluorescent dye injection into the acoustic ganglion of early chick embryos for selective tracing of auditory axon fibers in the nerve and hindbrain.
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
FAQs
The developing embryo can be reached by opening the shell or creating a window, allowing researchers to observe or alter embryonic tissues while development continues. This access links a specific intervention with later developmental changes, making it possible to examine how cells communicate, migrate, and contribute to organ formation without removing the embryo from its developmental environment.
Microsurgery, cell or tissue grafting, and substance injection create controlled changes in the developing embryo. Researchers can then examine how the altered cells or tissues behave and how development proceeds. These comparisons help identify developmental mechanisms, including interactions among cells and the processes that guide migration and organ formation.
Altering environmental conditions provides a way to test whether particular developmental outcomes depend on the surrounding conditions as well as on the embryo’s own tissues. Observing the resulting changes can clarify developmental mechanisms and support toxicology studies, where researchers investigate how substances or conditions affect continued embryonic development.
A typical workflow begins by accessing the embryo through the shell or a windowing technique. Researchers then observe the developing tissues and select an intervention, such as microsurgery, grafting cells or tissues, injecting a substance, or changing environmental conditions. Development continues afterward, allowing investigators to assess the consequences of the manipulation.
These approaches alter the embryo in different ways. Microsurgery directly changes embryonic structures, grafting introduces cells or tissues for study, and injection delivers a substance into the developing system. Selecting among them depends on whether the investigation focuses on tissue behavior, cellular interactions, gene function, developmental mechanisms, or responses to an introduced substance.
Researchers can apply the approach to embryology, genetics, developmental biology, regenerative research, toxicology, and comparative studies of vertebrate development. It is particularly useful when a study requires direct access to a developing embryo and controlled testing of gene function, tissue interactions, environmental effects, or the formation of embryonic organs.
Observations may show how embryonic cells communicate and migrate, how tissues participate in organ formation, or how development changes after a gene-related or environmental intervention. These outcomes provide evidence about developmental mechanisms and can support comparisons across vertebrate development, while also informing research on regeneration and the effects of potentially harmful conditions.