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Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo
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Chapters
- 00:04Title
- 00:49Micro-Injection Pipette Preparation
- 01:53Host Embryo Preparation
- 03:34Donor Tissue Isolation
- 04:44Donor Tissue Digestion
- 05:50In Vivo Injection
- 07:37Results: Representative Donor Myocyte-Implanted Embryonic Recipient Heart Imaging
- 08:14Conclusion
In this method, embryonic cardiac tissues are surgically microdissected, dissociated, fluorescently labeled, and implanted into host embryonic tissues. This provides a platform for studying the individual or tissue level developmental organization under ectopic hemodynamic conditions, and/or altered paracrine/juxtacrine environments.
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Keyword Extraction:
MicroinjectionIn Vivo ImplantationEmbryonic CardiomyocytesAvian EmbryoBiomechanicsParacrine EnvironmentsJuxtacrine InteractionsCellular PhenotypeExperimental EmbryologyGraphing ExperimentsInjection PressureInjection EquipmentGlass CapillariesSiliconizing AgentMicroinjection PipetteFertile Chicken EggsAlbumenTransparent TapeHamburger And HamiltonIndia InkHBSS
