Axonemal Dyneins
Axonemal Dyneins - Related Videos
08:09
Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
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Cited by 3 •
2019
The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.
10:52
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
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Cited by 19 •
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The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.
07:07
Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
0 Views •
Cited by 8 •
2016
Primary cilia influence various signaling pathways. The mammalian cochlea is ideal for examining planar cell polarity (PCP) signaling. Cilia dysfunction affects cochlear outgrowth, cellular patterning and hair cell orientation, readouts of PCP. Our goal is to analyze PCP signaling in mouse cochlea via phenotypic analysis, immunohistochemistry and scanning electron microscopy.
FAQs
ATP hydrolysis changes the activity of dynein arms so they generate directed movement against neighboring microtubule doublets. Because the arms are arranged within the axoneme, their force does not simply move the entire structure apart. Instead, the resulting microtubule sliding becomes mechanical input for coordinated ciliary or flagellar motion.
Structural constraints within the axoneme limit how far adjacent microtubule doublets can slide relative to one another. These restrictions redirect the motor-generated sliding into curvature, allowing successive regions of the axoneme to form bending waves. That conversion is essential because wave propagation, rather than unconstrained sliding, produces cell propulsion or fluid movement.
Neighboring microtubule doublets provide the interacting tracks against which dynein arms generate force. Their relative arrangement lets ATP-powered activity create directed sliding within the axoneme. The interaction therefore links molecular motor action to the larger mechanical behavior of cilia and flagella, including the coordinated movements needed for motility and fluid transport.
The same force-producing system can have different biological outcomes depending on the structure being moved. In flagella or motile cilia, axonemal dynein activity contributes to bending waves that propel cells. Across tissue surfaces, those waves move fluid instead. This makes the motor relevant to both organismal movement and surface-level transport.
Examining these motors helps connect molecular force generation with ciliary and flagellar roles in biology. Relevant processes include cell motility, fluid transport, reproduction, and respiratory clearance. Comparing how axonemal movement contributes to each context can reveal how the same underlying machinery supports distinct physiological functions.
Axonemal dyneins provide a mechanistic link between axonemal structure and the movement of cilia or flagella. Studying their function can therefore clarify how impaired ciliary activity relates to primary ciliary dyskinesia. The topic also helps researchers interpret consequences for processes that depend on motile cilia, including respiratory clearance and other forms of fluid transport.