Bile Acid Metabolism
Bile Acid Metabolism - Related Videos
09:21
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
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Cited by 8 •
2016
We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.
08:42
Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
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Cited by 8 •
2016
Tools to diagnose bile acid malabsorption and measure bile acid transport in vivo are limited. An innovative approach in live animals is described that utilizes combined proton (1H) plus fluorine (19F) magnetic resonance imaging; this novel methodology has translational potential to screen for bile acid malabsorption in clinical practice.
04:35
Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model
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2023
This video describes the detailed protocol for intestinal resection in a murine model. This technique provides insights into ileectomy-induced bile malabsorption, overaccumulation, and toxicity in the mouse intestine.
12:06
Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
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Cited by 7 •
2017
Sialic acid is a typical monosaccharide-unit found in glycoconjugates. It is involved in a plethora of molecular and cellular interactions. Here we present a method to modify cell surface sialic acid expression using metabolic glycoengineering with N-acetylmannosamine derivatives.
06:55
Ileectomy-induced Bile Overaccumulation in Mouse Intestine
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Cited by 4 •
2017
Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.
FAQs
Conjugation is a distinct hepatic processing step that occurs after cholesterol-derived molecules become primary bile acids and before their release into bile. Tracking whether molecules are unconjugated or linked to glycine or taurine helps distinguish stages of the pathway and connect liver processing with subsequent intestinal handling.
After bile acids assist lipid digestion, most are reabsorbed in the ileum and returned to the liver through enterohepatic circulation. This recycling allows them to continue participating in the pathway, while the fraction that is not reabsorbed undergoes elimination. Studying both routes clarifies how bile acids move through the body.
FXR and TGR5 link bile acid molecules with broader metabolic regulation through receptor activation. Their activity influences glucose, lipid, and energy homeostasis, allowing researchers to study bile acids as signaling inputs as well as digestion-related molecules. This receptor perspective connects molecular changes in the pathway with wider metabolic outcomes.
Intestinal microbes produce secondary bile acids, adding a biological transformation that complements hepatic production of primary bile acids. This makes the gut microbiome an active participant in the pathway rather than merely a site where bile acids are present. Comparing primary and secondary forms helps researchers examine microbial contributions to bile acid biology.
A pathway-focused investigation can examine hepatic synthesis from cholesterol, conjugation, release into bile, intestinal reabsorption, return through enterohepatic circulation, microbial production of secondary bile acids, and elimination. Including receptor activity adds the signaling dimension. This sequence relates chemical transformation, circulation, microbial contribution, and metabolic regulation within one biological system.
Analysis of this pathway provides a framework for investigating liver disease and metabolic disorders by linking hepatic processing, intestinal recycling, microbial transformation, and receptor-mediated regulation. It also supports research on gut microbiome interactions. Considering these connected stages helps scientists examine how digestion, signaling, and elimination contribute to broader biological outcomes.