Ileectomy-induced Bile Overaccumulation in Mouse Intestine

The overall goal of this surgery is to evaluate ileectomy-induced bile malabsorption, overaccumulation and toxicity in the mouse intestine. Diseases of the ileum as manifesting conditions such as Crohn's, cancer, autoimmune disorders or infection are important sources of morbidity and mortality. The development of this model that involves segmental removal of the ileum, we believe, can be leveraged for insights into biology as well as therapy.

The implications of this technique extend toward the therapy of bile acid diarrhea as ileectomy potentially induce bile acid overproduction by the liver and the malabsorption and overaccumulation by the intestine. Generally, individuals new to this method will struggle with the intestinal anastomosis which is the most critical and difficult portion of this surgery. Begin by confirming the appropriate level of sedation by toe pinch and applying depilatory cream to the mouse's abdomen.